中国兽医科学2018，Vol.48Issue(9)：1146-1151,6.DOI:10.16656/j.issn.1673-4696.2018.0146
猪源肠道益生乳酸菌的分离鉴定及其生物学特性的研究
Isolation and biological characteristics of swine probiotic lactic acid
摘要
Abstract
A strain of lactic acid bacteria(LYOOl) was isolated from healthy piglets , which was identified as Enterococcus faecium by Biolog automatic micro-analysis system. Compared its 16S rRNA gene with that of the Enterococcus faecium strain(accession No. GQ337884. 1) reported on Genbank,the strain was 99％ homology. In characteristics result,the strain reached to the stationary phase after growth for 6 h. It had a good resistance acid,salt and high temperature properties. In a drug sensitivity test,the strain LY001 was susceptible to penicillins,cephalosporins and quinolones, resistance to aminoglycosides,macrolides and tetracyclinesa. It had a capable of formation biofilm. The strain LY001 had a characteristics of suppressing growth of Escherichia coli,Salmonella and Staphylococcus. In a murine pathogenesis test,the strain had a satisfactory security. The above-mentioned results showed that the strain LY001 could be used for the selection of great probiotics.关键词
屎肠球菌/抗逆性/抑菌/致病性Key words
Enterococcus faecium/stress resistance/antimicrobial/pathogenesis分类
农业科技引用本文复制引用
陈松彪,陈慧敏,丁轲,廖成水,杨亚东,程相朝,张春杰,吴庭才..猪源肠道益生乳酸菌的分离鉴定及其生物学特性的研究[J].中国兽医科学,2018,48(9):1146-1151,6.基金项目
国家自然科学基金面上项目(31572489) （31572489）
