中国临床药理学杂志2018，Vol.34Issue(19)：2324-2326,2334,4.DOI:10.13699/j.cnki.1001-6821.2018.19.023
罗格列酮对大鼠重症急性胰腺炎并发肾损伤的保护作用
Protective effect of rosiglitazone in the treatment of the rats with severe acute pancreatitis and acute kidney injury
摘要
Abstract
Objective To investigate the protective effect of rosiglita-zone ( ROSI) in the treatment of the rats with severe acute pancreatitis ( SAP) and acute kidney injury ( AKI), and its influence on nuclear fac-tor related factor 2 /antioxidant response element (Nrf2/ARE) pathway. Methods Fourty-five SD rats were randomly divided into sham -ope-ration, model and test groups with 15 cases per group.The rat model of SAP combined with AKI was established by retrograde injection of 4%sodium taurocholate into the pancreaticobiliary duct . After abdominal cavity incision, Sham -operation group were only turned the duodenum and pancreas.30 min before the establishment of model , test group was given 10 mg· kg -1ROSI with intraperitoneal injection , sham-operation and model groups were given the same amount of 0.9%NaCl. The expression of serum amylase (AMS), blood urea nitrogen (BUN) and creatinine ( Cr) in serum were measured by au-tomatic biochemical analyzer.The expression of nuclear factor related factors 2 (Nrf2), heme oxygenase 1 (HO-1) and quinone oxidoreductase 1 (NQO1) in renal tissue were measured by Western blotting .Results The AMS of test, Sham-operation and model groups were (3007.45 ±127.53 ), (902.35 ±46.82 ) and (3562.86 ±165.37 )U· L-1, BUN were ( 10.62 ±1.64 ), ( 7.84 ±1.53 ) and ( 17.74 ±1.85 ) mmol· L-1, Cr were ( 102.83 ±11.26 ), (59.76 ±9.84 ) and ( 156.47 ±13.73 ) μmol· L-1, Nrf2 protein were ( 1.58 ±0.10 ), ( 0.57 ±0.05 ) and (1.05 ±0.07 ) , HO-1 protein were ( 2.29 ±0.14 ), ( 0.54 ±0.43 ) and (1.05 ±0.08 ), NQO1 protein were (1.56 ±0.09), (0.59 ±0.03) and (1.01 ±0.06 ), the differences were statistically significant between test group and model group ( all P<0.05).Conclusion ROSI has a renal protective effect on SAP combined with AKI in rats , and its mechanism may be related to the up regulation of HO -1 and NQO1 protein expression in Nrf2/ARE signaling pathway.关键词
罗格列酮/重症急性胰腺炎/急性肾损伤/核因子相关因子2/抗氧化反应元件信号信号通路Key words
rosiglitazone/severe acute pancreatitis/acute kidney injury/nuclear factor related factor 2/antioxidant response element signaling pathway分类
医药卫生引用本文复制引用
张跃明,黄坚,胡丽华..罗格列酮对大鼠重症急性胰腺炎并发肾损伤的保护作用[J].中国临床药理学杂志,2018,34(19):2324-2326,2334,4.基金项目
浙江省医药卫生科技计划课题资助项目(2016KYA158) （2016KYA158）
