中国临床药理学杂志2018，Vol.34Issue(19)：2342-2344,3.DOI:10.13699/j.cnki.1001-6821.2018.19.028
超高效液相色谱-质谱法测定小鼠眼角膜中环孢素的含量
Determination of the content of cyclosporine in mice cornea by ultra-performance liquid chromatography-mass spectrometry
庞文哲 1韩彬 1王强 1高燕霞 1张轶华 1崔晓燕1
作者信息
- 1. 河北省药品检验研究院 抗生素室,石家庄050011
- 折叠
摘要
Abstract
Objective To establish a ultra performance liquid chroma-tography-mass spectrometry to determine the content of cyclosporine in mice.Methods Mobile phase was methanol -water (90∶10), chroma-tographic column was: Hypersill C18column (100 mm×2.1 mm, 1.9 μm) and internal standard substance : Cyclosporine A, ion mode of ESI +was applied to determine cyclosporine and multi -response monitoring mode was used (MRM, cyclosporine m/z: 1224.9 ).Results Working solution over the concentration range of 10 ～200 ng · mL-1 (R＝0.9986) worked well for the determination of cyclosporine in mice cornea homogenate.The lowest concentration was 3.3 ng· mL-1.The average extraction recovery rate of cyclosporine in mice cornea reached almost 80%(n＝6), and RSD was less than 12%.The intra-precision was less than 10.12%and the inter -precision was less than 11.75%. Conclusion The established method was sensitivity , specific, repea-table for cyclosporine determination in mice cornea .关键词
环孢素/超高效液相色谱-质谱法/角膜Key words
cyclosporine/ultra -performance liquid chromatography -mass spectrometry/cornea分类
医药卫生引用本文复制引用
庞文哲,韩彬,王强,高燕霞,张轶华,崔晓燕..超高效液相色谱-质谱法测定小鼠眼角膜中环孢素的含量[J].中国临床药理学杂志,2018,34(19):2342-2344,3.
