吉林中医药2018，Vol.38Issue(10)：1181-1184,4.DOI:10.13463/j.cnki.jlzyy.2018.10.020
荣筋片对SAMP6鼠瘦素血清含量及骨组织蛋白表达的影响
Study on serum content and bone tissue protein expression of Leptin in SAMP6 mice by Rongjin tablets
摘要
Abstract
Objective To observe the effects of Rongjin tablet on serum leptin level and leptin protein expression in bone tissue of SAMP6 rats, and to provide a new idea for the study of the mechanism of Rongjin tablet in preventing osteoporosis. Methods Thirty 6 month old samp6 rats were randomly divided into Rongjin tablet group and blank control group according to body weight. Another 15 months with resistance to rapid aging SAMR1 mice, as homologous comparison control group. The Rongjin tablet group was given 3 mL solution, the blank control group was given 3 mL saline once a day for 12 weeks, the serum leptin content was detected by blood collection from eyeball, and the bone marrow of femur was taken to make smear. The expression of leptin protein in bone tissue was determined by immunohistochemistry. Results Serum Leptin was (0.323 8±0.142 1) ng/mL and Leptin protein expression of the bone was (0.21±0.13) ng/mL in Rongjin tablets group, serum Leptin was (0.351 9±0.225 7) ng/mL and leptin protein expression of the bone was (0.26±0.11) ng/mL in SAMRl control group, serum leptin was (0.173 7±0.036 9) ng/mL and Leptin protein expression of the bone was (0.17±0.08) ng/mL in the blank control group. Serum Leptin and protein expression of the blank control group was significantly low-er than the other two groups (P＜0.05). There was no obvious difference between Rongjin tablets group and SAMR1 control group (P＞0.05). Conclusion Rongjin tablets can inhibit the MSCs into adipose cells, promote the MSCs into osteoblasts and induction the adipocytes into osteoblasts. Rongjin tablets can improve Leptin content of the serum and the protein expression of bone tissue.关键词
荣筋片/骨质疏松症/瘦素Key words
Rongjin tablets/Osteoporosis/Leptin分类
医药卫生引用本文复制引用
李远栋,王平,张君涛,杨光,张雪竹..荣筋片对SAMP6鼠瘦素血清含量及骨组织蛋白表达的影响[J].吉林中医药,2018,38(10):1181-1184,4.基金项目
天津市应用基础与前沿技术研究计划(青年项目)(15JCQNJC12700) （青年项目）
王平劳模创新工作室·天津市教委项目(津教工2016:3号) （津教工2016:3号）
中医传承工作室·天津市卫计委项目(津卫中2017:193号) （津卫中2017:193号）
国家自然基金面上项目(81673994) (51573137). （81673994）
