陕西中医2018，Vol.39Issue(11)：1491-1496,1500,7.DOI:10.3969/j.issn.1000-7369.2018.11.001
扶正解毒化瘀颗粒对克雷伯杆菌肺炎所致多器官功能损伤小鼠心肌组织细胞因子及病理的影响研究
Effects of Fuzheng Jiedu Huayu granule on myocardial cytokines and pathology in myocardial tissue of multi-organ injury induced by klebsiella pneumonia in mice
摘要
Abstract
Objective :To reveal the possible mechanism of Fuzheng Jiedu Huayu granule on myocardial pro-tection of multiple organ injury induced by Klebsiella pneumoniae ,through cytokines and myocardial histopathology . Methods :128 KM mice were randomly divided into control group ,model group ,western medicine group and tradi-tional Chinese medicine group with 32 animals in each group .The multi-organ injury model was prepared by direct injection of Klebsiella pneumoniae by tracheal intubation method .8 mice were sacrificed on the 1st ,3rd ,5th ,and 7th days after modeling respectively ;the heart was taken out and divided into two parts .One part of the heart tissue was homogenized and the levels of TNF-α ,IL-1β ,IL-6 ,IFN-γ ,IL-10 were detected in different infection phases ;the oth-er part of the heart tissue was HE staining ,and the pathological changes of different infection phases were observed under light microscope.Results :Compared with the control group ,the cytokines level of TNF-α ,IL-1β ,IL-6 ,IFN-γ ,IL-10 in the myocardial tissue of the model group were significantly increased on the 1st and 3rd day after infec-tion ,and the pathological damage of myocardial tissue was obvious .Compared with the model group ,on the 1st day after infection ,the level of pro-inflammatory cytokines such as TNF-α ,IL-1β ,IL-6 and IFN-γ in myocardial tissue were significantly decreased ,and the level of anti-inflammatory cytokine IL-10 was obviously increased in the tradi-tional Chinese medicine group .Conclusion :Pro-inflammatory/anti-inflammatory cytokines are involved in the dam-age of myocardial tissue in pneumonia mice .Fuzheng Jiedu Huayu granule can reduce the expression levels of pro- inflammatory cytokines and improve the expression level of anti-inflammatory cytokine in myocardial tissue ,thereby reducing the degree of pathological damage of myocardial tissue and playing a protective role on myocardial tissue .关键词
肺炎/中医药疗法/肺炎克雷伯菌/@扶正解毒化瘀颗粒/动物实验/小鼠Key words
Pneumonia/traditional Chinese medicine/therapy Klebsiella pneumoniae/@Fuzheng Jiedu Huayu granule/Animal experimentation/Mice分类
医药卫生引用本文复制引用
李猛,王志梅,徐红日,王成祥,马战平..扶正解毒化瘀颗粒对克雷伯杆菌肺炎所致多器官功能损伤小鼠心肌组织细胞因子及病理的影响研究[J].陕西中医,2018,39(11):1491-1496,1500,7.基金项目
陕西省自然科学基金资助项目(2013JC2-08)陕西省重点研发计划项目(2017ZDXM-SF-109)陕西省中医药管理局科研项目(13-LC118)西安市科技局计划项目(SF1419-2) （2013JC2-08）
