陕西中医2018，Vol.39Issue(11)：1497-1500,4.DOI:10.3969/j.issn.1000-7369.2018.11.002
阳和汤对人乳腺癌MDA-MB-231细胞增殖、凋亡及其Akt/mTOR信号通路的影响
Effect of Yanghe decoction on proliferation ,apoptosis and Akt/mTOR signaling pathway in human breast cancer MDA-MB-231 cells
摘要
Abstract
Objective :MDA-MB-231 cells were intervened by Yanghe decoction medicated serum to explore the effects of Yanghe decoction on proliferation ,apoptosis and Akt/mTOR signaling pathway .Methods :Medicated serum (or positive drug ) was used to intervene MDA-MB-231 cells for 24 h ,48 h ,and 72 h ,and the cell OD value was determined by CCK-8 method to calculate inhibition rate .Apoptosis was detected by flow cytometry and the lev-els of Akt/mTOR signaling pathway proteins were detected by western blot after intervened by medicated serum (or positive drug) for 24 h.Results :After 24 ,48 ,and 72 h of intervention ,the inhibition rate of low dose group ,high dose group and positive drug group increased (P＜0 .01).After intervention of 24 h ,the apoptosis rate of low dose group ,high dose group and positive drug group increased ,Bcl-2/Bax value decreased ,and mTOR and Akt phospho-rylation rate of the low dose group ,high dose group and positive drug group decreased (P＜0 .05 or 0 .01).Conclu-sions :The effects of Yanghe decoction on breast cancer maybe inhibit Akt/mTOR signaling pathway to suppress proliferation of breast cancer cells and induce apoptosis .关键词
乳腺肿瘤/中医药疗法/阳和汤/治疗应用/动物实验/大鼠Key words
Breast neoplasms/traditional Chinese medicine therapy/Yang He Tang/therapeutic uses/Animal experimentation/Rats分类
医药卫生引用本文复制引用
黄芊,张扶莉,李雪莲,尚荣国,许崇文,钱建升,窦建卫..阳和汤对人乳腺癌MDA-MB-231细胞增殖、凋亡及其Akt/mTOR信号通路的影响[J].陕西中医,2018,39(11):1497-1500,4.基金项目
国家自然科学基金资助项目(81573983 ,81873311)陕西省重点研发计划项目(2017SF-109) （81573983 ,81873311）
