陕西中医2018，Vol.39Issue(11)：1646-1648,封3,4.DOI:10.3969/j.issn.1000-7369.2018.11.045
大黄活血化瘀作用的HPLC指纹图谱分析
HPLC fingerprint analysis of promoting blood circulation to remove blood stasis of traditional Chinese medicine rhubarb
摘要
Abstract
Objective :To study the characteristics of high performance liquid chromatography (HPLC) fin-gerprint of traditional Chinese medicine rhubarb promoting blood circulation to remove blood stasis .Methods :The effect of different extraction parts of rhubarb (systematic solvent extraction) on the hemodynamics ofrats with blood stasis was analyzed by rat experiment .The fingerprint of rhubarb promoting blood circulation to remove blood stasis site of actionwas analyzed by HPLC .Emodin ,chrysophanol and rhein were reference substances .Results :Compared with the blank control group ,the whole blood viscosity and erythrocyte aggregation rate were significantly higher in the model control group (P＜ 0 .05).Compared with the model control group ,the whole blood viscosity and erythro-cyte aggregation rate were significantly lower in rhubarbn-butanol site high and low dose groups and rhubarb 50%ethanol site high and low dose groups (P＜ 0 .05).The stability experiment showed that the relative standard devia-tion of relative retention time of main chromatographic peak and relative peak area consistency was lower than 2%, while the precision experiment showed the relative standard deviation was lower than 1%,and repeatability experim-entshow that the relative standard deviation was lower than 2% .The similarities of rhubarb medicinal materials were 0 .978 ,0 .995 ,0 .980 ,0 .956 ,0 .975 ,0 .991 ,0 .970 ,0 .974 ,0 .966 and 0 .993 ,respectively .Conclusion :Rhubarb promoting blood circulation to remove blood stasis sites include n-butanol and ethanol .The established method is stable and reproducible ,and it can be used for monitoring the effectiveness fingerprint of rhubarbpromoting blood circulation to remove blood stasis .关键词
大黄/活血祛瘀/色谱法,高压液相/@指纹图谱Key words
Rheum officinale/Blood-activating stasis-removing/Chromatography ,high pressure liquid/@Fingerprint分类
医药卫生引用本文复制引用
李付玻,王奎鹏,韩立,郭晓娟,房晓,何栋..大黄活血化瘀作用的HPLC指纹图谱分析[J].陕西中医,2018,39(11):1646-1648,封3,4.基金项目
河南省科技攻关项目( 122102310008) （ 122102310008）
