陕西中医2018，Vol.39Issue(11)：1649-1652,4.DOI:10.3969/j.issn.1000-7369.2018.11.046
不同炮制方法对补骨脂中抗骨质疏松成分含量水平的影响
Effects of different processing methods on thecontents of anti-osteoporosis components in fructus psoraleae
摘要
Abstract
Objective :To analyze the effects of different processing methods on the contents of anti-osteoporo-sis components in fructus psoraleae .Methods :Four kinds of anti-osteoporosis components (including psoralen , isopsoralen ,psoralidin ,bakuchiol) in fructus psoraleae were extracted by salt processed method ,Leigong method , wine steeping method and stir-frying method respectively .The contents were determined by high performance liquid chromatography (HPLC) ,and the effects of different processing methods on the contents were compared .Results :The results of HPLC electronic image showed that the compounds of psoralen ,isopsoralen ,psoralidin and bakuchiol were well separated within 30 min after sample introduction (relative resolution degrees were all＞1 .5).Within the corresponding concentration range ,the linear relationship between the above four compounds was good with r ≥0 . 9994 and peak area RSD≤2 .0% .Compared with the raw product ,Leigong method could increase 19 .52% of psor-alen and 14 .50% of isopsoralen .Salt processed method could increase 11 .51% of psoralidin .Wine steeping method could increase 7 .99% of psoralen and 11 .94% isopsoralen .Stir-frying method could make up 29 .45% of psoralidin . Conclusions :Leigong method for fructus psoraleae processing has greater effects on anti-osteoporosis indicatorscom-ponents than other methods .And it can significantly increase the contents of psoralen and isopsoralen .关键词
炮制(中药)/补骨脂/补骨脂素/色谱法,高压液相/中药化学成分Key words
Processing (TCD)/Psoralea corylifolia/Psoralen/Chromatography ,high pressure liquid/Chemical compositions (TCD)分类
医药卫生引用本文复制引用
张健,张伟..不同炮制方法对补骨脂中抗骨质疏松成分含量水平的影响[J].陕西中医,2018,39(11):1649-1652,4.基金项目
河南省教育厅科学技术研究重点项目(13B360981)河南省科技厅重点攻关项目(132102310261) （13B360981）
