生命科学研究2018，Vol.22Issue(5)：345-354,10.DOI:10.16605/j.cnki.1007-7847.2018.05.001
共生乳酸乳球菌加速黑腹果蝇发育
Commensal Lactococcus lactis Accelerates the Development of Drosophila melanogaster
摘要
Abstract
To isolate lactic acid bacteria from Drosophila melanogaster gut and investigate their effects on the developmental timing of its hosts,bacteria were isolated from the fly gut with MRS medium and further subjected to 16S rRNA gene sequence identification.The developmental timing and the growth rate of D.melanogaster were assayed.Immunofluorescent staining and real-time quantitative PCR were used to analyze the intestinal cell proliferation and activation of prothoracicotropic hormone (PTTH) and insulin signaling,respectively.Glucose oxidase method was used to determine the concentration of glucose.The results showed that a Lactococcus lactis strain was isolated from D.melanogaster and it efficiently colonized the gut of D.melanogaster.The bacterium facilitated the developmental timing of D.melanogaster by increasing the growth rate.At the cellular level,the number of mitotic cells in L.lactis-associated fly guts was approximately six times as much as that in germ-free (GF) sibling.And at the molecular level,the settled L.lactis activated the hormonal and insulin signals to accelerate the systemic growth of the fruit flies.In conclusion,L.lactis is one of the commensal bacteria of D.melanogaster,and it can stimulate the development of D.melanogaster through hormonal and insulin signals.关键词
乳酸乳球菌/黑腹果蝇/发育/生长速率/共生菌Key words
Lactococcus lactis/Drosophila melanogaster/development/growth rate/commensal bacteria分类
生物科学引用本文复制引用
王晓阳,张乐宵,张珂,李欣欣,苏琬真,张笑添,刘威..共生乳酸乳球菌加速黑腹果蝇发育[J].生命科学研究,2018,22(5):345-354,10.基金项目
国家自然科学基金资助项目(31501175) （31501175）
山西省大学生创新创业项目(2017536) （2017536）
山西医科大学汾阳学院科技发展重点基金资助项目(2018C02)The National Natural Science Foundation of China (31501175) （2018C02）
Shanxi Training Program of Innovation and Entrepreneurship for Undergraduates (2017536) （2017536）
the Key Fund of Shanxi Medical University Fenyang College Science and Technology Development (2018C02) （2018C02）
