医药导报2018，Vol.37Issue(11)：1321-1326,6.DOI:10.3870/j.issn.1004-0781.2018.11.006
二甲双胍对心肌纤维化模型大鼠的影响
Effect of Metformin on Myocardial Firbosis in Model Rats
摘要
Abstract
Objective To investigate the effects of metformin on myocardial fibrosis induced by isoproterenol(ISO) in rats and its mechanism. Methods A total of 48 SD male rats were randomly divided into four groups,normal control group,model control group,valsartan group and metformin group. Cardiac fibrosis models were induced in model group, Valsartan group and metformin group by subcutaneous injection of ISO 10 mg·kg-1·d-1in the 1stday, 5 mg·kg-1·d-1in the 2ndday, 3 mg·kg-1·d-1in the 3rd-10thday. Valsartan group and metformin group were fed valsartan 10 mg·kg-1, qd or metformin 100 mg·kg-1,bid for 28 days respectively.Rats were killed at the 3rdday,the 7thday,the 14thday and the 28thday.The value of HMI,LVMI,LVW/TL were measured,and pathological alteration were observed by HE staining and Masson staining,TGF-β and Smad3 protein expression in left ventricular myocardium were detected by western blotting,and IL-6 level in serum were detected by ELISA. Results The serum levels of IL-6 in control group, model group, valsartan group and metformin group were (59.18± 0.86) pg·mL-1, (71.90±1.35) pg·mL-1, (64.33±2.59) pg·mL-1and (65.45±1.92) pg·mL-1respectively on the 28th day. Compared with control group, the serum levels of IL-6 in model group were significantly increased(P<0.01), while their levels significantly reduced in rats received metformin (P<0.05). Conclusion Metformin may inhibit myocardial firbosis by TGF-β/Smads pathway.关键词
二甲双胍/心肌纤维化/转化生长因子β/Smads/白细胞介素-6Key words
Metformin/Myocardial firbosis/Transforming growth factor-β/Smads/IL-6分类
医药卫生引用本文复制引用
梁宇,赵春阳,杨立超,姜明燕..二甲双胍对心肌纤维化模型大鼠的影响[J].医药导报,2018,37(11):1321-1326,6.基金项目
辽宁省科技厅项目(2014226033) （2014226033）
