医药导报2018，Vol.37Issue(11)：1374-1376,3.DOI:10.3870/j.issn.1004-0781.2018.11.019
高效液相色谱法测定黑种草子中黑种草苷含量
Determination of Nigeglanoside in Seeds of Nigella glandulifera by HPLC
摘要
Abstract
Objective To establish a method for the determination of nigeglanoside in seeds of Nigella glandulifera. Methods The content of nigeglanoside was determined by HPLC.The separation was performed on a C18column ( YMC-Pack ODS-A,250 mm×4.6 mm,5 μm) with a gradient elution system of acetonitrile and 0.017 5 mol·L-1acetic acid solution at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 290 nm,and column temperature was 30 ℃. Results The linear range of nigeglanoside was 0.01-0.30 mg·mL-1(R2＝0.9991).The RSDs of precision,stability and repeatability were all less than 2%.The average recovery was 96.66% (RSD＝1.25%,n＝6). Conclusion The method is accurate and reproducible. It is effective in controlling the quality of seeds of Nigella glandulifera .关键词
黑种草子/黑种草苷/含量测定/色谱法,高效液相Key words
Seeds of Nigella glandulifera/Nigeglanoside/Content determination/Chromatography,high performance liquid分类
医药卫生引用本文复制引用
詹羽姣,李晨阳,胡旭,陈燕,赵军,徐芳..高效液相色谱法测定黑种草子中黑种草苷含量[J].医药导报,2018,37(11):1374-1376,3.基金项目
国家自然科学基金资助项目(81660655) （81660655）
新疆维吾尔自治区科技人才培养项目(QN2016YX0690) （QN2016YX0690）
