医药导报2018，Vol.37Issue(11)：1393-1396,4.DOI:10.3870/j.issn.1004-0781.2018.11.024
高效液相色谱法测定驱虫斑鸠菊中异绿原酸A和异绿原酸C含量
Determination of Isochlorogenic Acid A and Isochlorogenic Acid C in Vernonia Anthelmintica by High Performance Liquid Chromatography
摘要
Abstract
Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica. Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established. Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5. 825-69. 9 μg·mL-1, and the concentration of isochlorogenic acid C,was between 5.15-61.80 μg·mL-1and the peak area was good . The sample recovery rates of the two groups were 98.70%-101.92%(RSD＝1.04%,n＝9)、95.99%-102.52%(RSD＝1.90%,n＝9). Conclusion The method is simple,rapid, accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.关键词
驱虫斑鸠菊/异绿原酸A/异绿原酸C/色谱法,高效液相Key words
Vernonia anthelmintica/Isochlorogenic acid A/Isochlorogenic acid C/Chromatograph,high performance liquid分类
医药卫生引用本文复制引用
陈嘉媛,霍仕霞,闫明,宋志媛,斯拉甫·艾白..高效液相色谱法测定驱虫斑鸠菊中异绿原酸A和异绿原酸C含量[J].医药导报,2018,37(11):1393-1396,4.基金项目
新疆维吾尔自治区重点研发计划项目(2016B03038-3-3) （2016B03038-3-3）
