局解手术学杂志2018，Vol.27Issue(10)：707-710,4.DOI:10.11659/jjssx.03E018001
miR-128通过RECK靶向作用影响乳腺癌细胞的生物学行为
miR-128 affects biological behavior of breast cancer cells through the targeting of RECK
摘要
Abstract
Objective To investigate the biological behavior of breast cancer cells induced by miR-128 targeting RECK and its possible mechanism.Methods The expression of miR-128 in cell lines and transfection efficiency of lentiviral were detected by Western blot.Flow cytometry was used to detect the effect of miR-128 on the apoptosis of breast cancer cells.The dual luciferase assay was used to detect the in-teraction of miR-128 and RECK in breast cancer cells.Flow cytometry was used to detect the reversal effect of RECK on miR-128 in the cell cycle and colony formation of breast cancer cells.Results The expression of miR-128 in MCF-7 cells was 3.05 times that of NC cells,and the lentiviral transfection efficiency was obvious.miR-128 inhibits the growth of MCF-7 cells by inducing apoptosis and inhibiting cell cycle progression.RECK was the direct target of miR-128,and miR-128 directly regulated the expression of RECK.RECK ectopic recovery,miR-128 on breast cancer cell apoptosis and cell cycle inhibition had also been partially restored.Conclusion miR-128 plays an important role in the development and progression of breast cancer.It can interact with RECK to regulate the biological behavior of breast cancer cells,which suggests that miR-128 and RECK may be the potential therapeutic targets of breast cancer.关键词
乳腺癌/miR-128/RECK/细胞周期/凋亡/MCF-7细胞Key words
breast cancer/miR-128/RECK/cell cycle/apoptosis/MCF-7 cells分类
医药卫生引用本文复制引用
叶彦军,郝福军,陈巍,张玲..miR-128通过RECK靶向作用影响乳腺癌细胞的生物学行为[J].局解手术学杂志,2018,27(10):707-710,4.基金项目
宝鸡市科技计划项目(2017SH2) （2017SH2）
