中国临床药理学与治疗学2018，Vol.23Issue(10)：1081-1087,7.DOI:10.12092/j.issn.1009-2501.2018.10.001
硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析
Screening and bioinformatics analysis of differential microRNA in ni-troglycerin-tolerant rat aorta
摘要
Abstract
AIM: To screen the differential ex-pression of microRNA ( miRNA) in nitroglycerin-tol-erant rat aorta and to analyze their target genes and function. METHODS: Sprague Dawley ( SD) rats were treated with nitroglycerin ( 100 mg·kg-1· time-1for 3 days, three times a day, s. c). Then, miRNA sequencing technology and qRT-PCR were used to find differential miRNAs involved in nitro-glycerin-tolerant rats. Furthermore, bioinformatics analysis was performed to predict target genes regu-lated by these differential miRNAs, and to analyze the enriched gene ontology ( GO ) and signaling pathway. RESULTS:Compared with control group, the expression of 28 miRNAs changed significantly in nitroglycerin-tolerant group, of which 16 were known. 12 up-regulated and 4 down-regulated were obtained among the 16 miRNAs. The expression of miR-487b-3p and miR-503-5p were detected by qRT-PCR showed the similar trend as well as the se-quencing results. Bioinformatics analysis showed that the target genes of differential miRNAs were mainly enriched in protein binding and metabolic pathways. CONCLUSION: The expression of miR-NA in nitroglycerin-tolerant rat aorta changes signifi-cantly, and these differential miRNAs are involved in the development of nitroglycerin tolerance by reg-ulating their target genes.关键词
硝酸甘油/耐受/microRNA/生物信息学Key words
nitroglycerin/tolerance/microR-NA/bioinformatics分类
医药卫生引用本文复制引用
曾振华,刘建新,曹春芽,吴卫华..硝酸甘油耐受大鼠主动脉差异microRNA筛选与生物信息学分析[J].中国临床药理学与治疗学,2018,23(10):1081-1087,7.基金项目
湖南省教育厅科学研究项目(17B188) （17B188）
湖南医药学院青年优秀科研人才支持计划(2013-85) （2013-85）
