中国临床药理学与治疗学2018，Vol.23Issue(10)：1103-1108,6.DOI:10.12092/j.issn.1009-2501.2018.10.004
华蟾素对肺癌NCI-A549移植瘤裸鼠的抑制作用及机制研究
Inhibitory effect and mechanism of cinobufotalin on lung cancer NCI-A549 xenograft in nude mice
摘要
Abstract
AIM: To investigate the anti-tumor effect and mechanism of cinobufotalin on lung cancer cells NCI-A549. METHODS: Forty BALB/c nude mice were randomly divided into the normal group, the mock group, the cinobufotalin group and the DDP ( cisplatin) group. Body weight change, tumor inhibition rate, blood function, liver and kidney function of the mice in each group were detected. Western blot was used to investigate the expression of apoptosis-associated proteins. RESULTS:Cinobu-fotalin significantly inhibited the growth of A549 xenograft, but had no significant effect on the blood, liver and kidney function of the mice. The tumor-in-hibition rate of the cinobufotalin group and the DDP group was 58. 0% and 82. 3% , respectively. West- ern blot results showed that cinobufotalin significant-ly up-regulated the expression of Bax/Bcl-2, promo-ted the expression of cleaved-caspase-3, cleaved-PARP, and inhibited the expression of p-AKT (ser473). CONCLUSION: Cinobufotalin can sig-nificantly inhibit the growth of lung cancer A549 xenograft. Its molecular mechanism might be related to the inhibition of PI3K/AKT pathway activation. Based on this mechanism, cinobufotalin can acceler-ate cell apoptosis.关键词
华蟾素/肺癌/移植瘤/凋亡/PI3K/AKT通路Key words
cinobufotalin/lung cancer/xeno-graft/apoptosis/PI3K/AKT pathway分类
医药卫生引用本文复制引用
熊飞,施嫣嫣,沈久成,程洪兰,杭永付..华蟾素对肺癌NCI-A549移植瘤裸鼠的抑制作用及机制研究[J].中国临床药理学与治疗学,2018,23(10):1103-1108,6.基金项目
国家自然科学基金青年项目(81402830) （81402830）
