中国临床药理学与治疗学2018，Vol.23Issue(10)：1109-1115,7.DOI:10.12092/j.issn.1009-2501.2018.10.005
抗血小板溶栓素对大鼠脑缺血再灌注损伤保护作用机制研究
Study on the protective mechanism of anti-platelet thrombolysin against cerebral ischemia reperfusion injury in rats
摘要
Abstract
AIM: To investigate the protective mechanism of anti-platelet thrombolysin ( APT ) on cerebral ischemia reperfusion injury in rats. METHODS: The TLR4 siRNA in vivo transfection technique was used to reduce the TLR4 expression in the brain of rat. Wild-type and TLR4 down-regu-lated rats were randomly divided into six groups:sham group, model group, TAK-242 ( Toll-like re-ceprot 4 blocker) 2 mg/kg group, APT 0. 02 mg/kg, 0. 01 mg/kg, 0. 005 mg/kg group respectively with eight rats in each group. The rat focal ischemi-a-reperfusion injury model was established by liga-tion of middle cerebral artery occlusion ( MCAO ) . RhoA activity was measured using absorbance based G-LISA RhoA activation assay and the expression of TLR4, ROCK1/2 and p-JNK were determined by Western blot. RESULTS: The expression of TLR4 protein in TLR4 siRNA transfected rats brain tissue decreased significantly compared with that in control group rats. Compared with model group, APT could obviously decrease the TLR4, ROCK1/2 and p-JNK protein expression, inhibit the RhoA activity in wild rat brain; however, APT had no significant effect on RhoA activity, ROCK1/2 and p-JNK protein expres-sion in brain compared with model group in TLR4 siRNA transfected rats. CONCLUSION: The pro-tective mechanism of APT on cerebral ischemia reperfusion injury in rats is mainly related to the in-hibition of TLR4/RhoA/ROCK signaling pathway.关键词
抗血小板溶栓素/siRNA在体转染/Toll样受体/RhoA/ROCK信号通路Key words
anti-platelet thrombolysin/siRNA transfection in vivo/toll like receptor 4/RhoA/ROCK signaling pathway分类
医药卫生引用本文复制引用
罗胜勇,贾德武,李小羿,戴向荣..抗血小板溶栓素对大鼠脑缺血再灌注损伤保护作用机制研究[J].中国临床药理学与治疗学,2018,23(10):1109-1115,7.基金项目
安徽省自然科学基金资助项目(1608085MH161) （1608085MH161）
