中国临床药理学与治疗学2018，Vol.23Issue(10)：1126-1131,6.DOI:10.12092/j.issn.1009-2501.2018.10.007
仙灵骨葆联合益生菌对骨质疏松性骨折大鼠的愈合作用
Healing effect of Xianling Gubao plus probiotics on osteoporotic frac-ture rats
摘要
Abstract
AIM: To study the healing effect of Xianling GuBao ( XLGB) plus probiotics on osteopo-rotic fracture rats. METHODS: Fifty female Spra-gue-Dawley rats were randomly divided into five groups: fracture control group, osteoporotic fracture model group, XLGB treatment group, probiotics treatment group and XLGB combined probiotics treatment group, 10 rats in each group. In addition to the conventional fracture control group, the other groups were treated with bilateral ovaries to prepare osteoporosis model. After 6 weeks, each group was prepared for right middle femoral fracture model. Then, Saline, XLGB (250 mg·kg-1·d-1) or pro-biotics ( 12. 5 g·kg-1·d-1) were intragastrically administered separately. The right femur specimens of each group were taken 5 weeks after surgery. The bone mineral density was measured bone mineral density scanner, CR and Micro-CT were used to ob-serve femur related parameters in rats. The bone al-kaline phosphatase ( BALP) , tartrate resistant acid phosphatase ( TRACP) contents were measured by ELISA kit, calcium( Ca) concentration was measured by biochemical analyzer. HE staining was used to observe the pathological changes of periosteum in fractures. RESULTS: Compared with the osteopo-rotic fracture group, the BALP level was increased, the TRACP level was decreased, the bone density and the amount of callus were increased, the frac-ture line was blurred, the number and thickness of the trabecular bone were increased, and the trabecu-lar space was decreased in XLGB and probiotic group, and XLGB combined probiotic group ex-pressed the most obvious effect of treatment ( P <0. 05, P < 0. 01 ). CONCLUSION: Both XLGB and probiotics showed the therapeutic effect on os-teoporotic fractures, and the combination of drugs has a better effect on osteoporotic fracture.关键词
仙灵骨葆/益生菌/骨质疏松性骨折/骨源性碱性磷酸酶/抗酒石酸酸性磷酸酶Key words
Xianling Gubao/probiotics/osteo-porosis fracture healing/bone alkaline phosphatase/tartrate resistant acid phosphatase分类
医药卫生引用本文复制引用
邓祖跃,黄俊俊..仙灵骨葆联合益生菌对骨质疏松性骨折大鼠的愈合作用[J].中国临床药理学与治疗学,2018,23(10):1126-1131,6.基金项目
浙江省自然科学基金一般项目(LY14H270003) （LY14H270003）
