中国临床药理学杂志2018，Vol.34Issue(20)：2402-2405,4.DOI:10.13699/j.cnki.1001-6821.2018.20.003
高迁移率蛋白N2对人膀胱移行细胞癌及裸鼠移植瘤的影响
Effect of high mobility group chromosomal protein N2 ( HMGN2 ) on growth of bladder cancer cells and xenograft tumor in nude mice
摘要
Abstract
Objective The inhibitory effect of high mobility group chro-mosomal protein N2(HMGN2) on growth of human bladder cancer T24 cells and xenograft tumor in nude mice .Methods In vitro, using thia-zole blue method (MTT) to detect the effect of 0, 3, 5, 7, 9 μg· mL-1 HMGN2 on the activity of T24 cells.Flow cytometry was used to detect the inhibitory effect of 0, 1, 3, 5 μg· mL-1HMGN2 on T24 cells.The expressions of apoptotic proteins Bcl -2, caspase -3, p53 and Bax in cells were detected by Western blot .In vivo, xenograft tumor in nude mice was constructed and divided into blank group , control group and ex-perimental group.Phosphate buffer solution ( PBS ), cis -dichlorodia-mineplatinum (DDP) and HMGN2 were respectively injected around the tumor tissue.All nude mice were sacrificed on post -transplantation 20 d, tumor tissue was removed.The effects of necrosis and apoptosis was analyzed by Hematoxylin and eosin (HE) staining and TUNEL.Re-sults MTT results indicated that the growth of T24 cells was inhibited by 3, 5, 7, 9 μg· mL-1HMGN2, and the inhibition rate increased significantly with the increase of HMGN 2 concentra-tion and the incubation time.Flow cytometry results showed that the total apoptosis rate of 1, 3, 5 μg· mL-1HMGN2 in T24 cells were ( 13.18 ±0.83 )%, (25.79 ±1.22 )%, ( 31.48 ±1.82 )%, had significant difference with 0 μg· mL-1HMGN2 (P＜0.01 ).HE staining and TUNEL detection results of tumor tissues in nude mouser showed that compared with blank group , tumor tissues in experimental group and control group showed obvious necrosis and apoptosis.Conclusion HMGN2 may inhibit the proliferation of T24 cells and the growth of xenograft tumor in nude mice by promoting the necrosis of tumor tissue and inducing apoptosis .关键词
高迁移率蛋白N2/T24细胞/移植瘤/膀胱癌Key words
high mobility group chromosomal protein N2/T24 cell/xenograft tumor/carcinoma of urinary bladder分类
医药卫生引用本文复制引用
蒋萍,岳进巧,李静,董震宇,杨旭,雷冬玉,吴桂霞..高迁移率蛋白N2对人膀胱移行细胞癌及裸鼠移植瘤的影响[J].中国临床药理学杂志,2018,34(20):2402-2405,4.基金项目
国家自然科学基金资助项目(81660480) （81660480）
新疆维吾尔自治区高校科研计划基金资助项目(XJEDU2014I019) （XJEDU2014I019）
新疆昌吉职业技术学院科研计划基金资助项目(CJZY2017027) （CJZY2017027）
