中国临床药理学杂志2018，Vol.34Issue(20)：2406-2408,3.DOI:10.13699/j.cnki.1001-6821.2018.20.004
ATF5调节乳腺肿瘤细胞的生长和侵袭性的机制研究
ATF5 in regulating the growth and invasiveness of breast tumor cells
摘要
Abstract
Objective To investigate the mechanism of ATF5 regulate the growth and invasiveness of breast tumor cells .Methods The T47D breast cancer cells was used , siRNA1 group was transfected ATF5 siRNA1 sequence, siRNA2 group was transfected siRNA1 sequence, control group was transfected ATF5 control sequence.The expression of ATF5 mRNA were detected by real -time polymerase chain reaction (PCR), the expression of ATF5 protein were detected by Western blot , the survival rate of cell were detected by clones , and the proliferation ability of the cells were detected by cell count kit ( CCK)-8.Results After transfection of 24, 48 and 72 h, the expression of ATF5 in siRNA1 group and siRNA2 group were lower than that in control group (P＜0.05).The growth rate of group siRNA1 at 24, 48 and 72 h were significantly lower than that of control group ( P ＜0.05 ) .The survival rates of siRNA1, siRNA2, control groups were 0.98 ±0.02, 0.98 ±0.03 and 0.98 ±0.02 , and there were no significant difference compared among three groups ( P ＞0.05 ) .Conclusion The decreased expression of ATF5 can reduce the growth and invasiveness of breast cancer cells and block the cell cycle.关键词
乳腺癌/细胞周期/侵袭性Key words
breast cancer/cell cycle/invasiveness分类
医药卫生引用本文复制引用
赵丹,杨林,朱剑梅,金科..ATF5调节乳腺肿瘤细胞的生长和侵袭性的机制研究[J].中国临床药理学杂志,2018,34(20):2406-2408,3.基金项目
四川省卫生和计划生育委员会科研课题基金资助项目(140082) （140082）
