中国临床药理学杂志2018，Vol.34Issue(20)：2409-2412,4.DOI:10.13699/j.cnki.1001-6821.2018.20.005
Slit2/Robo1的在宫颈癌细胞异常低表达与对细胞凋亡的影响
Abnormal low expression of Slit2/Robo1 in cervical cancer cells and its effect on cell apoptosis
摘要
Abstract
Objective To study the expression of Slit2 and its receptor Robo1 in human cervical carcinoma and its effect on the apoptosis of cer-vical cancer cells and its underlying mechanism .Methods Real-time polymerase chain reaction ( PCR) and Western blot were used to detect the mRNA and protein of Slit2, Robo1 and Bcl-2 in 20 cases of normal cervical epithelium (NCE) and 40 cases of cervical intraepithelial neo-plasia (CIN), 60 cases of cervical squamous cell carcinoma (SCC) tis-sue expression. HeLa cells were transfected with siRNA ( silencing group), plasmid ( overexpression group ) and transfected pCMV -HA plasmid (pCMV-HA control) and unrelated siRNA sequences ( negative control ) by the transfection reagent Lipo-fectamine2000.Cells were harvested after a specific time for subsequent experiments .After staining with Annexin V/PI, the effects of Robo1 overexpression and Slit2 silencing on apoptosis were detected by flow cytometry .The clea-vage form of PARP-1 and the expression of Bcl -2 were analyzed by immunoblotting .Results The relative expression levels of Slit2 mRNA in NCE, CIN and SCC were 2.48 ±0.36 , 1.78 ±0.22 , 1.45 ±0.17 , Robo1 mRNA were 2.69 ±0.31 , 1.94 ±0.21, 1.48 ±0.15 , and Bcl-2 mRNA were 1.61 ±0.21, 2.22 ±0.23 , 2.85 ±0.28 , all with significant difference (all P＜0.05).The relative expression levels of Robo1 mRNA in overexpression group and pCMV-HA control group were 3.48 ±0.51 and 1.00 ±0.14, with significant difference (P＜0.05).The early apoptosis, late apoptosis and total apoptotic rate in overexpression group were ( 7.81 ±0.80 )%, ( 14.22 ±1.80 )% and (23.11 ±2.02 )%, had significant difference with those in pCMV -HA group, which were ( 2.60 ±0.82 )%, (7.33 ±0.81)%and (9.91 ±1.72)%(P＜0.05).Slit2 in silent group and negative control group were 1.00 ±0.11 and 0.51 ±0.12 , with significant difference (P＜0.05).The early apoptosis, late apoptosis and total apoptosis rate in negative control were (4.91 ±1.12)%, (7.90 ±1.01)%, (12.80 ±1.70)%; had significant difference with those in silent group, which were (2.82 ±1.01)%, (5.13 ±2.10)%, (7.92 ±1.90)%(P＜0.05).Conclusion Slit2/Robo1 is abnormally expressed in cervical cancer tissues , which is beneficial to resist apoptosis and play an im-portant role in the development of cervical cancer .关键词
宫颈癌/宫颈上皮内瘤变/B淋巴细胞瘤-2/细胞凋亡/分子机制Key words
cervical cancer/cervical intraepithelial neoplasia/B -cell lymphoma -2/cell apoptosis/molecular mechanism分类
医药卫生引用本文复制引用
黄小莉,曲军英,郭玮..Slit2/Robo1的在宫颈癌细胞异常低表达与对细胞凋亡的影响[J].中国临床药理学杂志,2018,34(20):2409-2412,4.基金项目
福建省卫生厅青年基金资助项目(2010115) （2010115）
