中国临床药理学杂志2018，Vol.34Issue(20)：2421-2423,3.DOI:10.13699/j.cnki.1001-6821.2018.20.008
乌灵胶囊对小鼠认知功能的影响
Effect of Wuling Capsule on cognitive function of mice
摘要
Abstract
Objective To investigate the effect of Wuling Capsule on cognitive function of Alzheimer disease model mice (5×FAD) and the mechanism of connexin 43 ( Cx43).Methods The 5xFAD mice were randomly divided into model group ( n =10 ) and experimental group (n=10),healthy mice as normal group (n=10).At 4 months old,the mice in the experimental group were given Wuling Capsule by gastrointesti-nal administration once a day at 9:00 am at a dose of 150 mg· kg-1· d-1 for 2 months, which was weighed to 40 mL in a 0.5% CMC-Na solu-tion.The normal group and model group received regular diet .The cog-nitive function of mice was evaluated by Water maze and Y maze .Immu-nohistochemical method was used to detect the expression of Cx 43 protein in the brain of mice.Results After the administration , the escape la-tency of the experimental group and model group on the third day after training was (58.0 ±1.3), (63.0 ±1.2) s, and the difference was sta-tistically significant ( P ＜0.05 ) . The quadrant retention time (32.0 ±1.5)s and cross-platform times (4.6 ±1.1) s of the experi-mental mouse platform were significantly higher than the model group (19.0 ±1.7), (1.9 ±1.2 ) s ( all P ＜0.05 ) .After two months of administration, the number of erroneous responses (3.9 ±0.5) times in the experimental group to passive stimuli for safe escape was significantly lower than that in the model group (6.3 ±1.6 ) times ( P＜0.05) .Conclusion Wuling Capsule can improve the cognitive function by increasing the secretion of Cx43 protein.关键词
乌灵胶囊/缝隙连接蛋白43/认知功能/神经胶质细胞Key words
Wuling Capsule/connexin 43/cognitive function/glial cell分类
医药卫生引用本文复制引用
邹小冬,张震中,程有根,李中春,王海,胡智伟..乌灵胶囊对小鼠认知功能的影响[J].中国临床药理学杂志,2018,34(20):2421-2423,3.基金项目
浙江省中医药科学研究基金资助项目(2015ZB010) （2015ZB010）
