中国临床药理学杂志2018，Vol.34Issue(20)：2424-2426,3.DOI:10.13699/j.cnki.1001-6821.2018.20.009
蒿本内酯对三硝基苯磺酸诱导溃疡性结肠炎大鼠的保护作用及机制
Protective effect and mechanism of ligustilide on ulcerative colitis induced by trinitrobenzene sulfonic acid in rats
摘要
Abstract
Objective To study the protective effect and its mechanism of ligustilide on ulcerative colitis -induced by trinitrobenzene sulfonic acid (TNBS) in rats.Methods The rats were randomly divided into 5 groups(n=12):blank group, model group, low-, middle-, and high-dose experimental groups ( ligustilide 10, 20, 40 mg· kg -1) .The model of ulcerative colitis was established by injected TNBS 100 mg· kg -1and 50% ethanol into colon of rats except for the blank group, then drugs was given intragastrically for 14 d since the third day after modeling.The index of disease activity was observed .The levels of interleukin-4 ( IL -4 ), interleukin -10 ( IL -10 ) and tumor necrosis factor-α( TNF-α) in serum were determined by enzyme -linked im-munosorbent assay .Results The disease activity indices of the blank group, the model group and the high , medium and low dose groups were (0.33 ±0.14 ), ( 1.83 ±0.49 ), ( 0.94 ±0.27 ), ( 1.18 ±0.33 ), (1.35 ±0.30 ) point.The levels of IL -4 in serum of these 5 groups were (118.27 ±15.23 ), ( 51.35 ±9.80 ), (93.04 ±13.26), (86.31 ±11.92) and (73.09 ±10.57)ng· L-1.The levels of IL-10 in serum of these 5 groups were (85.63 ±10.05 ), (38.13 ±5.86 ), ( 70.92 ±8.87 ), ( 62.09 ±9.28 ) and (53.38 ±7.41 ) ng· L-1.The levels of TNF -α in serum of these 5 groups were ( 43.63 ±5.97 ), ( 81.25 ±11.36 ), ( 52.89 ±6.22 ), (63.30 ±7.83 ), and (72.85 ±10.73 ) ng· L-1, respectively.Compared with the blank group , the differences of all indexes in the model group were statistically significant (all P＜0.01).Compared with the model group , all indexes in the high and middle dose experimental groups were statistically significant (P＜0.05, P＜0.01).Conclusion Ligus-tilide had a protective effect on rat with ulcerative colitis , and its mechanism may be related to up -regulation of IL-4, IL-10 and down-regulation of TNF-α.关键词
蒿本内酯/溃疡性结肠炎/细胞因子/炎症Key words
ligustilide/ulcerative colitis/cytokine/inflammation分类
医药卫生引用本文复制引用
李燕,张瑞丽,范磊,马姝丽..蒿本内酯对三硝基苯磺酸诱导溃疡性结肠炎大鼠的保护作用及机制[J].中国临床药理学杂志,2018,34(20):2424-2426,3.基金项目
河南省医学科技攻关计划基金资助项目(201502326) （201502326）
