摘要
Abstract
Objective To investigate the effect of Panax notoginseng saponins ( PNS ) on the expression of basic fibroblast growth factor (b-FGF) and vascular endothelial growth factor (VEGF) in rats with traumatic fracture of the lower extremities.Methods A model of trau-matic fracture of the lower extremities was constructed in 45 cases of SD rats and they were randomly divided into experimental -L/-H group and model group.The experimental -L/-H group received intraperito-neal injection of 100, 200 mg· kg -1of PNS, respectively, while the model group was given intraperitoneal injection of the equal amount of normal saline respectively.All groups were given treatment once daily for 30 days.The levels of D-dimer (D-D) and von willebrand factor (vWF) in rats plasma were detected at 2 h, 3 d and 10 d after drug intervention respectively , the expression of b -FGF and VEGF protein in the bone tissue of rats were detected by immunohistochemical method at 10 d, 20 d and 30 d after drug intervention respectively .Results At 2 h after PNS treatment, the plasma levels of D-D in model group and experimental -L/-H group were ( 0.64 ±0.06 ), ( 0.42 ±0.08 ), ( 0.31 ±0.05 ) pg· mL-1,respectively, the plasma levels of vWF were (115.89 ±12.06)%, (89.47 ±8.49)%, (72.89 ±9.52)%, respectively.At 3 d after PNS treatment, the plasma levels of D-D in model group and experimental -L/-H group were (0.68 ±0.05 ), ( 0.55 ±0.08 ), ( 0.44 ±0.07 ) pg· mL-1, respectively, the plasma levels of vWF were (106.25 ±11.21)%, (85.12 ±6.49)%, (68.15 ±8.21)%, respectively.At 10 d after PNS treatment, the plasma levels of D -D in model group and experimental -L/-H group were ( 0.35 ±0.04 ), ( 0.24 ±0.07 ), (0.21 ±0.06 ) pg· mL-1, respectively, the plasma levels of vWF were ( 76.59 ±6.45 )%, ( 70.11 ±5.41 )%, (65.12 ±6.05)%, respectively.The differences were statistically significant among all groups (P＜0.05).At 30 d after PNS treatment, the relative expression of b -FGF in the bone tissue of model group and experimental -L/-H group were 0.26 ±0.02 , 0.33 ±0.03 , 0.35 ±0.03 , respectively;the relative expression of VEGF were 0.27 ±0.01 , 0.34 ±0.03 , 0.37 ±0.06 , respectively.The difference between the experimental -L/-H group and the model group was statistically significant ( P ＜0.05 ) .Conclusion PNS exerted protective effect on vascular endothelium by inhibiting blood hypercoagulability after traumatic fracture ; meanwhile, it could increase the expression of b -FGF and VEGF in bone tissue and promote fracture healing .关键词
骨折/三七总皂甙/碱性成纤维细胞因子/血管内皮生长因子/愈合Key words
fracture/Panax notoginseng saponins/basic fibroblast growth factor/vascular endothelial growth factor/healing分类
医药卫生
