摘要
Abstract
Objective To establish a HPLC-MS/MS method to deter-minate the concentration of linagliptin in human plasma .Methods The samples were treated by protein precipitation method .The chromato-graphic column was AQ-C18HP (2.1 mm×100.0 mm,3.0 μm), mo-bile phase was 100%water contained with 10 mmol· L-1ammonium ac-etate and 0.1% formic acid -95% acetonitrile contained with 0.1%formic acid, flow rate was 0.40 mL· min -1, column temperature was 40℃, injection volume was 15.0 μL, mobile phase. The specificity, standard curve and lower limit of quantitation , precision and recovery, matrix effect and stability of the method were investigated .Results The standard curve of metformin was y=9.35 ×10 -2x +1.88 ×10 -4( r =0.998 2), and linagliptin had good linear relationship in 0.05 -25.00 ng· mL-1.The limit of quantification was 0.05 ng· mL-1.The intra-day RSD and inter-day RSD were less than 10.0%, respectively.The recovery rate of extraction was over 103.8%.Conclusion The method is simple, rapid, accurate and sensitive , which is suitable for the deter-mination of linagliptin in human plasma .关键词
利格列汀/液相色谱-串联质谱法/血浆药物浓度Key words
linagliptin/HPLC-MS/MS/plasma concentration分类
医药卫生
