中国医学创新2018，Vol.15Issue(28)：15-18,4.DOI:10.3969/j.issn.1674-4985.2018.28.004
携带人VEGF165、Ang1基因的AAV-2促血管新生研究
Study on Function of AAV-2 with VEGF and Ang1 in Promoting Angiogenesis
摘要
Abstract
Objective:To evaluate the potentiality of the recombined adeno-associated virus-2(adeno-associated virus,AAV-2) carrying the human vascular endothelial growth factor-165(vascular endothelial growth factor,VEGF165) and angiopoietin-1(angiopoietin-1) gene in promoting vascular regeneration, by direct injection into muscle tissue of the male New Zealand rabbits.Method:32 male New Zealand white rabbits were randomly divided into four groups,8 rabbits in each group.The virus vector carrying human VEGF165 gene AAV-2-hVEGF165, carrying the human Ang1 gene AAV-2-hAng1,carrying beta-galactosidase(Gal) gene rAAV-2-LacZZ and carrying both human Ang1 and VEGF165 gene AAV-2-hAng1/VEGF165,which directly injected into muscle tissue of the male New Zealand rabbit, vascular regeneration were analyzed by visual observation and immunohistochemical assay 4 weeks later.Result:The muscle tissue injected AAV-2/hAng1/VEGF165 presented rich density and wider diameter of regenerated vascular,which was better than the AAV-2-hVEGF165,AAV-2-hAng1,AAV-2-LacZ in promoting vascular regeneration.Conclusion:Efficient expression and extraordinary vascular regeneration of AAV-2 carrying both human Ang1 and VEGF165 gene is obversed in the muscle tissue, AAV-2-hAng1/VEGF165 not only promote the angiogenesis,arteriogenesis and restoration of blood flow of ischemic limb,but also reduce the permeability of blood vessels,the therapeutic effectiveness is best among other groups.关键词
血管生成素1/血管内皮生长因子/腺相关病毒/血管新生Key words
Angiopoietin-1/Vascular endothelial growth factors/Adeno-associated virus/Vessels renewable引用本文复制引用
蔡冠军,谭最,章长杰..携带人VEGF165、Ang1基因的AAV-2促血管新生研究[J].中国医学创新,2018,15(28):15-18,4.基金项目
国家自然科学基金资助项目(30271265) （30271265）
