中国医学创新2018，Vol.15Issue(30)：13-16,4.DOI:10.3969/j.issn.1674-4985.2018.30.004
非酒精性脂肪肝患者血清中exosomes的水平及意义
Level and Significance of Serum Exosomes in Patients with Nonalcoholic Fatty Liver Disease
摘要
Abstract
Objective:To investigate the level of serum exosomes in patients with nonalcoholic fatty liver disease(NAFLD) and its effect on lipid metabolism in hepatic cells.Method:Western-blotting,transmission electron microscope and Nanosight were used to identify the character of exosomes.The protein content in serum exosomes in 19 cases of NAFLD(NAFLD group) and 18 cases of healthy controls(healthy control group) were detected by BCA protein assay.10 μg of exosomes were co-cultured with HepG2 cells.After 48 h,the cells were harvested,and the expression of total cholesterol(TC) and total triglyceride(TG) was detected by GPO-PAP method.The phosphorylation level of adenosine monophosphate activated protein kinase(AMPK) was detected by Western-blotting.Result:The expression of CD9,CD63 and CD81 were high in exosomes,which was round or elliptical with a diameter of 40-210(110.00±21.36)nm.The protein level of exosomes in NAFLD group was higher than that of healthy control group(P<0.05).After co-culture of exosomes with HepG2 cell,the expression of TC and TG significantly increased compared with those in control group(P<0.05).Conclusion:Exosomes can affect the activity of AMPK pathway and play an important role in the lipid metabolism of hepatocyte,which promote the development of NAFLD.关键词
非酒精性脂肪肝/exosomes/AMPK/脂肪代谢Key words
Nonalcoholic fatty liver disease/Exosomes/AMPK/Lipid metabolism引用本文复制引用
石柳,汤建华,温建军,曹德清,刘雪,林晔,张静智..非酒精性脂肪肝患者血清中exosomes的水平及意义[J].中国医学创新,2018,15(30):13-16,4.基金项目
江西省卫生计生委科技计划项目(20187226) （20187226）
