摘要
Abstract
Objective:To explore the effect of U0126 on proliferation and apoptosis in acute leukemia cell HL-60.Method:U0126 cell was cultured with 0,5,10,20 μmol/L dose of U0126 for 24,48,72 h.Cell viability was measured by MTT assay.Cell morphology was detected by Hoechst stainning.Cell proliferation was detected by EdU staining.Cell cycle was measured by flow cytometry.The expression of Proliferating Cell Nuclear Antigen(PCNA),Survivin,p21,p27 and the level of extracellular regulated protein kinases(ERK1/2) phosphorylation were measured by Western blot.Result:HL-60 cell viability was reduced for treatment with 5, 10,20 μmol/L dose of U0126 for 24,48,72 h(P<0.01),time- and dose-dependent manner(r=0.421, 0.478).5,10,20 μmol/L dose of U0126 reduced cell proliferation rate(P<0.01),made cell nucleus dense and thick,made cell cycle arrested in the G1 phase(P<0.01),down-regulated the expression of PCNA, Survivin and p-ERK1/2(P<0.01),up-regulated the expression of p21 and p27(P<0.01).Conclusion:U0126 can significantly inhibit the proliferation of HL-60 cells and induce apoptosis.关键词
U0126/急性白血病/HL-60/增殖/凋亡Key words
U0126/Acute leukemia/HL-60/Proliferation/Apoptosis
