中国中医药信息杂志2018，Vol.25Issue(11)：31-35,5.DOI:10.3969/j.issn.1005-5304.2018.11.008
基于Klotho研究三七总皂苷对大鼠肾缺血-再灌注损伤的保护机制
Study on Protective Mechanism of Panax Notoginseng Saponins on Rats with Renal Ischemia Reperfusion Injury Based on Klotho
摘要
Abstract
Objective To investigate the effects of Panax Notoginseng saponins (PNS) on protein expression of Klotho in rats with renal ischemia reperfusion injury; To discuss its protective mechanism for model rats. Methods Experimental rats were randomly divided into sham-operation group, model group, positive medicine group, PNS high-, medium- and low-dosage groups. Each administration group was given relevant medicine for gavage, once a day. Renal ischemia reperfusion injury model was established. Rats were sacrificed by taking blood from abdominal aorta after 4 hours of modeling. Serum levels of blood urea nitrogen (BUN), creatinine (SCr), malondialdehyde (MDA) content in kidney tissue, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity were measured. HE staining was used to observe the morphological changes of renal tissue. The protein expressions of Klotho and NF-κB p65 were measured by immunohistochemical method. Results Compared with the sham-operation group, the levels of BUN and SCr in the model group increased significantly (P<0.05); protein expression of Klotho in renal tissue decreased and the protein expression of NF-κB p65 increased (P<0.05). Compared with the model group, the expression of Klotho increased but protein expression of NF-κB p65 decreased in each administration group (P<0.05); Compared with the positive medicine group, the expression of Klotho in PNS high-dosage group increased but protein expression of NF-κB p65 decreased (P<0.05). The protein expression of NF-κB p65 was negatively related to protein expression of Klotho (r=-0.895, P<0.05). Conclusion PNS can inhibit oxidative stress and anti-inflammatory effects through upregulating protein expression of Klotho, and reduce the protein expression of NF-κB p65, and thus exerts renal protective effects.关键词
肾缺血-再灌注损伤/氧化应激/Klotho/三七总皂苷/大鼠Key words
renal ischemia reperfusion injury/oxidative stress/Klotho/Panax Notoginseng saponins/rats分类
医药卫生引用本文复制引用
庄高建,胡鸿运,杨英,唐紫荆,孙轩龙,刘春燕,唐群..基于Klotho研究三七总皂苷对大鼠肾缺血-再灌注损伤的保护机制[J].中国中医药信息杂志,2018,25(11):31-35,5.基金项目
国家自然科学基金(81503442) （81503442）
湖南中医药大学大学生研究性学习和创新性实验计划课题(2017-39) （2017-39）
湖南省教育厅科研项目(17B197) （17B197）
