中国中医药信息杂志2018，Vol.25Issue(11)：73-76,4.DOI:10.3969/j.issn.1005-5304.2018.11.016
基于含量分析探讨黄芪-葛根药对不同配比的差异
Discussion on Difference of Different Compatibility Ratios of Astragali Radix and Puerariae Lobatae Radix Based on Content Analysis
摘要
Abstract
Objective To study the contents of puerarin in decoction with different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix; To verify the rationality of compatibility ratios of Huangqi Gegen Decoction and Yu y e Decoction; To provide references for clinical medication. Methods Different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix were set as 0:1, 1:1, 2:1, 3:1, 4:1, 1:2, 1:3, and 1:4. SinoChrom ODS-BP (4.6 mm × 250 mm, 5 μm) was used as the chromatographic column to detect the contents of puerarin;methanol-0.1% phosphoric acid (35:65) was used as the mobile phase with 0.5 mL/min flow rate; sample volume was 20 μL; the wavelength was 250 nm. Results The regression equation of puerarin was Y=66 449.269 1X-175 665.663 1 (r=0.999 6) in the range of 5 to 300 μg/mL, showing a good linear relationship. The repeatability, stability and recovery rate were fine as well. The contents of puerarin were (2.506 7±0.025 8)%, (2.526 7±0.071 2)%, (2.863 3± 0.086 4)%, (2.956 7±0.119 6)%, (2.835 0±0.078 7)%, (2.480 0±0.072 4)%, (2.530 0±0.064 8)%, and (2.183 3±0.128 9)% in different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix with 0:1, 1:1, 2:1, 3:1, 4:1, 1:2, 1:3, and 1:4, respectively. Conclusion When the compatibility ratios of Astragali Radix and Puerariae Lobatae Radix are 2:1. 3:1, and 4:1, the contents of puerarin in decoction are the highest. This study verifies that the compatibility ratios of Astragali Radix and Puerariae Lobatae Radix in classical prescriptions are rational, which can be the optimal compatibility ratios in clinic.关键词
黄芪-葛根药对/配伍比例/葛根素/含量分析/高效液相色谱法Key words
Astragali Radix and Puerariae Lobatae Radix/compatibility ratios/puerarin/content analysis/HPLC分类
医药卫生引用本文复制引用
佟智斌,谭红,尹聪,邱路雅,袁丽君,涂星..基于含量分析探讨黄芪-葛根药对不同配比的差异[J].中国中医药信息杂志,2018,25(11):73-76,4.基金项目
湖北省教育科学规划重点课题(2016GA024) （2016GA024）
湖北民族学院大学生创新创业训练计划(2016CX087) （2016CX087）
