组织工程与重建外科杂志2018，Vol.14Issue(5)：245-249,5.DOI:10.3969/j.issn.1673-0364.2018.05.002
明胶/姜黄素纳米纤维电纺膜皮下环境构建组织工程软骨的可行性研究
The Construction of Tissue Engineered Cartilage by Using Gelatin/Curcumin Nanofibres Membranes in Subcutaneous Model
KANG BO KYOUNG 1陈维明 2周广东 2曹德君3
作者信息
- 1. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 2. 200011 上海市 上海组织工程研究重点实验室
- 3. 200241 上海市 组织工程研究国家工程中心
- 折叠
摘要
Abstract
Objective To explore the feasibility of constructing tissue engineered cartilage by using gelatin/curcumin nanofibres membranes as scaffold in subcutaneous model. Methods The seed cells were obtained from rabbit ear cartilage. After in vitro culture and expansion, cartilage was constructed with gelatin nanofibers membrane (control group) and gelatin/curcumin nanofibers membrane (experimental group) as scaffolds. Gross observation was performed after the material was implanted into the back of the BALB/c nude mice; SEM observation of the material and cell-material complex were all performed; The cell proliferation rate of the cell-material complex was measured; The cell-material complex cultured in vitro for 1 week was implanted into the back of BALB/c nude mice. Histological examination was performed 3 and 12 weeks after implantation, and cartilage formation at different time points was compared. Results Gross observation showed that compared with the control group, the vascularization of the tissue around in the experimental group was significantly inhibited; The SEM observation showed that the chondrocyts in the two groups adhered well; Growth curve of the chondrocyts showed that curcumin had no significant effect on cell growth; Histological observation showed that the cell-material complex of both groups had cartilage lacuna formation after implanted for 3 and 12 weeks, but compared with the control group, the material in experimental group showed obvious absorption, and the cartilage matrix was thicker and more homogenization. Conclusion Gelatin/curcumin membranes could be successfully used as scaffold in the construction of tissue engineering cartilage in subcutaneous animal model. It is a kind of scaffold material with potential application prospect.关键词
组织工程化软骨/纳米纤维电纺膜/姜黄素/抗血管化Key words
Tissue engineered cartilage/Nanofibres membranes/Curcumin/Anti-vascularization分类
医药卫生引用本文复制引用
KANG BO KYOUNG,陈维明,周广东,曹德君..明胶/姜黄素纳米纤维电纺膜皮下环境构建组织工程软骨的可行性研究[J].组织工程与重建外科杂志,2018,14(5):245-249,5.
