广西医科大学学报2018，Vol.35Issue(12)：1609-1614,6.DOI:10.16190/j.cnki.45-1211/r.2018.12.001
Mechanisms of inhibition by aspirin of endometrial carcinoma cell proliferation,migration and invasion
Mechanisms of inhibition by aspirin of endometrial carcinoma cell proliferation, migration and invasion
摘要
Abstract
Objective:To investigate the effects and mechanisms of aspirin on the proliferation,migration and invasion of endometrial carcinoma HEC-1A cell lines.Methods:HEC-1A cells were cultured to the exponential phase and treated with different concentrations of aspirin (0.625 mmol/ L,1.25 mmol/L,2.5 mmol/L,5 mmol/L and 10 mmol/L) for 24 to 120 hours.Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) assay.The migration and invasion of HEC-1A cells were detected by transwell assay.The protein expressions of vascular endothelial growth factor (VEGF) and ascular endothelial growth factor receptor 2 (VEGFR-2) in HEC-1A cells were determined by western blotting.Results:MTT results showed that aspirin inhibited the growth and proliferation of endometrial cancer cells in concentration and time-dependent manner.Aspirin had a significant inhibitory effect on the migration and invasion of HEC-1A cells (P＜0.05).In addition,aspirin obviously suppressed concentration-dependently the expression levels of VEGF and VEGFR-2 (P ＜ 0.05).Conclusion:Aspirin could inhibit the proliferation,migration and invasion of endometrial cancer cells.The anti-tumor mechanism of aspirin might be related to the inhibition of tumor angiogenesis via blocking the VEGF/VEGFR-2 signaling pathway.关键词
endometrial carcinoma/aspirin/proliferation/invasion/vascular endothelial growth factorKey words
endometrial carcinoma/aspirin/proliferation/invasion/vascular endothelial growth factor分类
医药卫生引用本文复制引用
Hongyan Chen,Lili Li,Qin Luo,Jiangtao Fan..Mechanisms of inhibition by aspirin of endometrial carcinoma cell proliferation,migration and invasion[J].广西医科大学学报,2018,35(12):1609-1614,6.基金项目
This work was supported by The Natural Science Fund of Guangxi Province (No.2016GXNSFAA380258) （No.2016GXNSFAA380258）
Innovation Project of Guangxi Graduate Education (No.YCSW2017104) （No.YCSW2017104）
The Health and Family Planning Commission's Self-financed Scientific Research Project of Guangxi (No.Z2016282). （No.Z2016282）
