肿瘤药学2018，Vol.8Issue(6)：851-855,5.DOI:10.3969/j.issn.2095-1264.2018.06.04
载阿霉素碳量子点纳米粒的制备及其对人肺癌细胞A549的体外抗肿瘤作用研究
Preparation of Doxorubicin-loaded Carbon Quantum Dot Nanoparticles and Its Anti-tumor Effect on Human Lung Cancer Cell A549 in Vitro★
李阳 1朱建华 1侍慧慧 1巩亚翔 1刘阳 1徐群为1
作者信息
- 1. 南京医科大学药学院,江苏 南京,211166
- 折叠
摘要
Abstract
Objective To prepare drug-loaded carbon quantum dots nanoparticles and investigate their anti-tumor effects in vitro. Methods The carbon-carbon quantum dots (CDs-COOH) were synthesized in one step by hydrothermal method. The amino group of doxo-rubicin (DOX) was covalently linked to the carboxyl group of CDs-COOH through an amide reaction to synthesize CDs-DOX loaded carbon quantum dot nanoparticles. CDs-COOH was studied by fluorescence spectrophotometer, infrared spectrometer, ultraviolet spectrophotometer and transmission electron microscopy. CDs-DOX was characterized by infrared spectroscopy, ultraviolet spectrophotometer and particle size analyzer. The toxicity of CDs-DOX to human lung cancer cell A549 was tested by MTT assay. Results The transmission electron microscopy showed that the CDs-COOH was spherical and the average particle size was about 5 nm. The results of fluorescence spectrophotometer fur-ther verified the uniform size of CDs-COOH. The UV-visible spectrophotometer and infrared spectroscopy results also confirmed the suc-cessful preparation of CDs-COOH and CDs-DOX. MTT experiments showed that CDs-DOX had high cytotoxicity to A549 when compared with free DOX. Conclusion The drug-loaded carbon quantum dots nanoparticles CDs-DOX were successfully prepared. It had anti-tumor effects in vitro and showed great potential in the development of anti-tumor drug delivery.关键词
碳量子点/阿霉素/纳米粒/体外抗肿瘤/MTT/人肺癌细胞A549Key words
Carbon quantum dots/Doxorubicin/Nanoparticles/Anti-tumor in vitro/MTT/Human lung cancer A549分类
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李阳,朱建华,侍慧慧,巩亚翔,刘阳,徐群为..载阿霉素碳量子点纳米粒的制备及其对人肺癌细胞A549的体外抗肿瘤作用研究[J].肿瘤药学,2018,8(6):851-855,5.
