动物营养学报2019，Vol.31Issue(2)：746-756,11.DOI:10.3969/j.issn.1006-267x.2019.02.031
浓缩棉籽蛋白替代鱼粉对卵形鲳鲹幼鱼生长性能、血清生化指标、肝脏抗氧化指标及胃肠道蛋白酶活性的影响
Effects of Fish Meal Replacement by Concentrated Cottonseed Protein on Growth Performance, Serum Biochemical Indices, Liver Antioxidant Indices and Gastrointestinal Tract Protease Activities of Juvenile Trachinotus ovatus
摘要
Abstract
This experiment was conducted to study the effects of fish meal replacement by concentrated cotton-seed protein on growth performance, serum biochemical indices, liver antioxidant indices and gastrointestinal tract protease activities of juvenile Trachinotus ovatus. A total of 720 juvenile Trachinotus ovatus with body weight of ( 25.5±0.5) g were selected and divided into 6 groups with 3 replicates per group and 40 fish per replicate. Fish in the control group were fed a basal diet which used fish meal as the main protein source, and others in the replacement groups were fed iso-nitrogen and iso-lipid diets which used the concentrated cotton-seed protein replaced 12%, 24%, 36%, 48% and 60% fish meal, respectively. The experiment lasted for 8 weeks. The results showed as follows: 1) compared with the control group, the final weight, weight gain rate and specific growth rate of replacement groups were no significant difference ( P>0. 05) , the final weight, weight gain rate and specific growth rate of 36% replacement group were significantly higher than those of 60% replacement group ( P<0.05) . The survival rate of control group and 48% replacement group was significantly higher than that of 12%, 24% and 36% replacement groups ( P<0.05). There were no significant differences in feed intake, feed coefficient and protein efficiency ratio among all groups ( P>0.05) . 2) The hepatopancre-as somatic indices of 12% replacement group was significantly higher than that of 24% replacement group ( P<0.05) , the condition factor of 24% replacement group was significantly higher than that of the control group ( P<0.05), the crude protein content of 36%, 48% and 60% replacement groups was significantly higher than that of control group and 12% replacement group ( P<0.05) , the crude ash content of 60% replacement group was significantly higher than that of the control group ( P<0. 05) . There were no significant differences in moisture and crude lipid contents among all groups ( P>0.05) . 3) The serum total protein content of 12% re-placement group was significantly lower than that of control group and 36%, 48% replacement groups ( P<0.05) ; the serum triglyceride content of 12% replacement group was significantly lower than that of other groups ( P<0.05) , the serum triglyceride content of 36% replacement group was significantly higher than that of other groups ( P<0.05) ; the serum glucose content of the control group was significantly higher than that of 60% replacement group ( P<0.05) ; the serum cholesterol content of control group and 12% replacement group was significantly lower than that of other groups ( P<0.05). 4) There were no significant differences in liver malondialdehyde content and serum total superoxide dismutase activity among all groups ( P>0.05) . The liver glutathione peroxidase activity of the control group was significantly lower than that of other groups except 60% replacement group ( P<0.05) , the liver total superoxide dismutase activity of the control group was significant-ly lower than that of 36%, 48% replacement groups ( P<0. 05) . 5) The pepsin activity of 36%, 48% and 60% replacement groups was significantly higher than that of 12% replacement group ( P<0.05) . There were no significant differences in mid-intestine chymotrypsin activity among all groups ( P>0.05) , the anterior in-testine and posterior intestine chymotrypsin activity of 24% and 36% replacement groups was significantly high-er than that of the control group ( P<0.05) . Under the conditions of this experiment, the specific growth rate is use to multivariate regression model analysis, the optimal replacement percentage of concentrated cottonseed protein replaced fishmeal in juvenile Trachinotus ovatus diet is 26%.[ Chinese Journal of Animal Nutrition, 2019, 31(2) :746-756]关键词
浓缩棉籽蛋白/鱼粉/卵形鲳鲹/生长性能/血清生化指标Key words
concentrated cottonseed protein/fish meal/Trachinotus ovatus/growth performance/serum bio-chemical indices分类
农业科技引用本文复制引用
申建飞,陈铭灿,刘泓宇,谭北平,董晓慧,杨奇慧,迟淑艳,章双,王华朗..浓缩棉籽蛋白替代鱼粉对卵形鲳鲹幼鱼生长性能、血清生化指标、肝脏抗氧化指标及胃肠道蛋白酶活性的影响[J].动物营养学报,2019,31(2):746-756,11.基金项目
国家自然科学基金项目(31772864) （31772864）
湛江市科技攻关项目(2016A03011) （2016A03011）
广东海洋大学创新强校项目(2015KTSCX055) （2015KTSCX055）
大学生创新创业项目(CXXL2017002) （CXXL2017002）
