动物营养学报2019，Vol.31Issue(2)：757-763,7.DOI:10.3969/j.issn.1006-267x.2019.02.032
仔猪断奶后1周内血清生化指标、十二指肠形态结构及空肠中钠/葡萄糖共转运载体1与二肽转运载体1基因表达量的变化
Changes in Serum Biochemical Indices, Duodenal Morphology, Expression Levels of Sodim-Gluose Transporter 1 and Peptidetransporter 1 Genes in Jejunum of Piglets at the First Week Post-Weaning
摘要
Abstract
The experiment was conducted to investigate the changes in serum biochemical indices, duodenal morphology and expression levels of sodim-gluose transporter 1 ( SGLT1) and peptidetransporter 1 ( PepT1) genes in jejunum of piglets at pre-weaning and the first week post-weaning. A total of 55 piglets at 20 days of age were selected, five piglets were slaughtered at 1 d pre-weaning, and the remained 50 piglets ( weaned at 21 days of age) were randomly divided to 5 groups ( pens) with 10 piglets per pen. One piglet per pen was sacri-ficed on d 2, 4 and 8 post-weaning, respectively. The results showed as follows: 1) compared with pre-wea-ning, the alkaline phosphatase activity in serum on d 2 post-weaning was significantly decreased ( P<0.05) and was very significantly decreased on d 4 post-weaning ( P<0.01) ; serum potassium ion content was significantly increased on d 4 and 8 post-weaning ( P<0.05) ; serum urea nitrogen content was very significantly increased on d 2 post-weaning ( P<0.01) and significantly decreased on d 8 post-weaning ( P<0.05); serum immuno-globulin G content was significantly decreased after weaning ( P<0.05) . 2) Compared with pre-weaning, the duodenal villus height was significantly decreased on d 2 post-weaning ( P<0.01) and d 4 post-weaning ( P<0.05) , crypt depth in duodenum was significantly enhanced on d 2 and 4 post-weaning ( P<0.05), and the ra-tio of villus height to crypt depth was also significantly decreased on d 2 and 4 post-weaning ( P<0.05) . 3) Compared with pre-weaning, the expression levels of SGLT1 gene in jejunum was also significantly decreased after weaning ( P<0.05) ; the expression level of PepT1 gene in jejunum on d 4 and 8 post-weaning was signif-icantly decreased ( P<0.05) . These results suggest that weaning on the first week causes a certain stress on physiological functions of piglets. Weaning reduces the serum immunoglobulin G content, changes the duode-nal morphology, decreases the expression levels of SGLT1 and PepT1 genes in jejunum, which may affect the digestion and absorption of carbohydrates and proteins, especially on d 2 and 4 post-weaning.[ Chinese Journal of Animal Nutrition, 2019, 31(2) :757-763]关键词
仔猪/血清生化指标/肠道形态/基因表达/断奶前后Key words
piglets/serum biochemical indices/intestinal morphology/gene expression/pre- and post-wea-ning分类
农业科技引用本文复制引用
李梦云,聂芙蓉,程伟,朱宽佑,郭良兴,张天勇,刘延贺..仔猪断奶后1周内血清生化指标、十二指肠形态结构及空肠中钠/葡萄糖共转运载体1与二肽转运载体1基因表达量的变化[J].动物营养学报,2019,31(2):757-763,7.基金项目
郑州市1125创新领军人才项目(2016XL003) （2016XL003）
校科技创新团队(HUAHE2015005) （HUAHE2015005）
