动物营养学报2019，Vol.31Issue(2)：764-774,11.DOI:10.3969/j.issn.1006-267x.2019.02.033
白术多糖可能通过 Toll样受体4信号通路缓解环磷酰胺诱导的雏鹅肝脏损伤
Polysaccharide of Atractylodes Macrocephala Koidz May Alleviates Liver Injury Induced by Cyclophosphamide Via Toll-Like Receptor 4 Signaling Pathway in Goslings
摘要
Abstract
This trial was conducted to evaluate the effects and possible mechanism of polysaccharide of Atrac-tylodes macrocephala Koidz ( PAMK) on liver injury induced by cyclophosphamide ( CTX) in goslings. A to-tal of 180 healthy one-day old Magang goslings were randomly divided into 3 groups ( control group, CTX group and PAMK+CTX group) with 3 replicates per group and 20 geese per replicate in a single-factor com-pletely randomized experimental design. Goslings in PAMK+CTX group were fed the basal diet supplemented with 400 mg/kg PAMK from 4 days of age to the end of the trial. On 19, 20 and 21 days of age, goslings in the control group were intramuscular injected 0.5 mL normal saline, and goslings in CTX group and PAMK+CTX group were intramuscular injected 40 mg/kg BW CTX solution, respectively. The trail lasted for 28 days. The results showed as follows: 1) compared with the control group, the liver index of control group was sig-nificantly increased ( P<0.05) , and the gallbladder index was significantly decreased ( P<0.05) . The liver in-dex and gallbladder index of PAMK+CTX group had no significant difference compared with the control group ( P>0.05) . 2) The liver 8-hydroxy-deoxyguanosine content of CTX group and PAMK+CTX group was signif-icantly higher than that of the control group ( P<0.05) . 3) The CTX could cause liver pathological damages, DNA oxidative damage of hepatic cells and some lesions in the liver, such as tissue fibrosis, inflammatory small focus, cholestasis et al. The PAMK could alleviate extent of damage of liver tissue, reduce the number of necrotic hepatocytes. 4) Compared with the control group, the liver malondialdehyde ( MDA) content of CTX group was significantly increased ( P<0.05) , and the activities of superoxide dismutase ( SOD) , glutathione peroxidase ( GSH-Px) , catalase ( CAT) , inducible nitric oxide synthase ( iNOS) in liver were significantly decreased ( P<0.05) . The MDA content and GSH-Px, iNOS activities in liver of PAMK+CTX group had no significant difference compared with the control group ( P>0.05) . 5) The mRNA relative expressions of Toll-like receptor 4 ( TLR4) , tumor necrosis factor receptor-associated factor 6 ( TRAF6) , mitogen activated pro-tein kinase kinase 1 ( MEKK1) , mitogen-activated protein kinase kinase 3 ( MKK3) , p38 mitogen-activated protein kinase ( p38MAPK) , inhibitor of nuclear factor-κB α ( IκBα) and inhibitor of nuclear factor kappa-B kinase α ( IKKα) in liver of CTX group were significantly lower than those of the control group ( P<0.05) . The mRNA relative expressions of TLR4, myeloid differentiation primary response ( MyD88 ) , MEKK1, MKK3, mitogen-activated protein kinase kinase 6 ( MKK6 ) , inhibitor of nuclear factor kappa-B kinase ε ( IKKε) and IκBαin liver of PAMK+CTX group were significantly higher than those of CTX group ( P<0.05), the mRNA relative expressions of MyD88 and MKK6 in liver were significantly higher than those of the control group ( P<0.05) , and the mRNA relative expressions of TRAF6, MEKK1, MKK3 and p38MAPK in liver were significantly lower than those of the control group ( P<0.05) . These results suggested that PAMK may regulate liver injury induced by CTX in goslings via TLR4 signaling pathway.[ Chinese Journal of Animal Nutrition, 2019, 31(2) :764-774]关键词
白术多糖/雏鹅/肝脏损伤/氧化应激/Toll样受体4信号通路Key words
PAMK/goslings/liver injury/oxidative stress/Toll-like receptor 4 signaling pathway分类
农业科技引用本文复制引用
钱隆,刘洋,李冰心,付晶,李婉雁,曹楠,田允波,许丹宁..白术多糖可能通过 Toll样受体4信号通路缓解环磷酰胺诱导的雏鹅肝脏损伤[J].动物营养学报,2019,31(2):764-774,11.基金项目
2017年"攀登计划"广东省大学生科技创新培育专项(pdjh2017b0254) （pdjh2017b0254）
广东省"创新强校工程"基础研究重大项目(2017KZDXM046) （2017KZDXM046）
广东省科技计划项目(2017B020202010) （2017B020202010）
