动物营养学报2019，Vol.31Issue(2)：858-864,7.DOI:10.3969/j.issn.1006-267x.2019.02.042
沙葱总黄酮对过氧化氢诱导的红细胞氧化损伤的保护作用
Protective Effects of Total Flavonoids from Allium mongolicum Regel on Oxidative Damage of Erythrocytes Induced by Hydrogen Peroxide
摘要
Abstract
The aim of this study was to investigate the protective effect of total flavonoids from Allium mongoli-cum Regel on oxidative damage of erythrocytes induced by hydrogen peroxide ( H2O2 ) in vitro. Based on the optimal H2O2 concentration and induction time required for the establishment of erythrocytes oxidative damage model and the safe concentration range of total flavonoids from Allium mongolicum Regel, the present experi-ment were divided into control group, H2O2 damage group and different concentrations of total flavonoids from Allium mongolicum Regel groups ( the final concentrations of total flavonoids from Allium mongolicum Regel in cell suspension were 30, 130, 260, 390 and 520 μg/mL, respectively) , each group had 9 replicates. Subse-quently, the effects of total flavonoids from Allium mongolicum Regel on hemolysis rate of erythrocytes, melondialdehyde ( MDA) content, activities of superoxide dismutase ( SOD) , catalase ( CAT) and glutathi-one peroxidase ( GSH-Px) were evaluated. The results showed that total flavonoids from Allium mongolicum Regel could significantly inhibit the hemolysis of erythrocytes induced by H2O2 oxidative stress ( P<0. 05 ) , which the effect was dose dependent. Compared with the H2O2 damage group, 130, 260 and 520 μg/mL total flavonoids from Allium mongolicum Regel significantly reduced the erythrocytes MDA content ( P<0.05) , and significantly increased the activities of SOD, CAT and GSH-Px in erythrocytes ( P<0.05). To sum up, total flavonoids from Allium mongolicum Regel can inhibit the hemolysis induced by H2O2 oxidative stress, reduce the production of peroxide products and increase the activities of antioxidant enzymes, thereby protect erythro-cytes from damages of oxidative stress.[ Chinese Journal of Animal Nutrition, 2019, 31(2) :858-864]关键词
沙葱/黄酮/红细胞/抗氧化Key words
Allium mongolicum Regel/flavonoids/erythrocytes/antioxidant分类
农业科技引用本文复制引用
张艳梅,敖长金,萨茹丽,赵亚波,王翠芳,哈斯额尔敦..沙葱总黄酮对过氧化氢诱导的红细胞氧化损伤的保护作用[J].动物营养学报,2019,31(2):858-864,7.基金项目
国家自然科学基金青年科学基金项目(31601961) （31601961）
国家自然科学基金地区基金项目(31260558) （31260558）
内蒙古自治区自然科学基金项目[2016BS(LH)0301] （LH）
