动物营养学报2019，Vol.31Issue(2)：900-911,12.DOI:10.3969/j.issn.1006-267x.2019.02.047
脱氧雪腐镰刀菌烯醇与黄曲霉毒素 B1单一及联合染毒对小鼠肠道菌群的影响
Effects of Single and Combined Exposure of Deoxynivalenol and Aflatoxin B1 on Intestinal Microflora in Mice
摘要
Abstract
The present experiment was conducted to evaluate the effects of single and combined exposure of de-oxynivalenol (DON) and aflatoxin B1 ( AFB1 ) on intestinal microflora diversity and species abundance in mice. Ninety-six Kunming mice ( male, 18-day-old) were randomly divided into 4 groups with 24 mice in each group. Mice in control group were fed physiological saline by gavage. Mice in DON group were fed 500 μg/kg DON by gavage. Mice in AFB1 group were fed 200 μg/kg AFB1 by gavage. Mice in DON+AFB1 group were fed 500 μg/kg DON+200 μg/kg AFB1 by gavage. The trial was conducted for 45 d. On the days 0 ( before the experiment) and 45 of the experiment, the fresh dejections in the rectum from each group ( each group was randomly taken 3 samples) were randomly taken under aseptic conditions, and then the changes of the structure and composition of the microbial community were analyzed using Illumina HiSeq high-throughput sequencing technology. The results showed that compared with the control group, the abundances of Bacteroidetes and Bacteroidia in DON, AFB1 and DON+AFB1 groups was significantly reduced ( P<0.01), and the abundance of Bacteroidia in DON+AFB1 group was significantly lower than that in DON and AFB1 groups ( P<0. 01) . Compared with the control group, the abundance of Deferribacteres in DON, AFB1 and DON+AFB1 groups was significantly reduced ( P<0. 01 ) , while the abundance of Clostridia in DON, AFB1 and DON+AFB1 groups was significantly increased ( P<0.01) . Moreover, the abundance of Spirochaetes in DON and DON+AFB1 groups was significantly higher than that in control group ( P<0.01) . In conclusion, single and combined exposure of DON and AFB1 all can alter the abundances of intestinal microflora, decrease the abundances of beneficial bacteria, and increase the abundances of harmful bacteria. DON and AFB1 exhibit a significant inter-action with synergistic effects.[ Chinese Journal of Animal Nutrition, 2019, 31(2) :900-911]关键词
脱氧雪腐镰刀菌烯醇/黄曲霉毒素B1/联合毒性/高通量测序/肠道菌群Key words
deoxynivalenol/aflatoxin B1/combined toxicity/high-throughput sequencing/intestinal micro-flora分类
农业科技引用本文复制引用
刘锦芳,李玉,吴金节,王希春,储小燕,刘秦,张娅菲,朱雷,张咪,陈铃霞,刘明达,冯士彬..脱氧雪腐镰刀菌烯醇与黄曲霉毒素 B1单一及联合染毒对小鼠肠道菌群的影响[J].动物营养学报,2019,31(2):900-911,12.基金项目
安徽省生猪产业技术体系资助项目(AHCYJSTX-05) （AHCYJSTX-05）
