动物营养学报2019，Vol.31Issue(2)：922-929,8.DOI:10.3969/j.issn.1006-267x.2019.02.049
一种基于酶标仪检测天然植物复方多糖中多糖含量的方法
Determination Method of Polysaccharide Content in Natural Plant Polysaccharide Compound Based on Microplate Reader
摘要
Abstract
This study aims to investigate the feasibility of measuring the polysaccharide content by a full-wave-length microplate reader instead of the ultraviolet spectrophotometer, and to verify the reliability of the results. First, the glucose content of the glucose standard solution was detected by ultraviolet spectrophotometer and full-wavelength microplate reader, respectively, to confirm the feasibility of measuring the polysaccharide con-tent by a full-wavelength microplate reader instead of the ultraviolet spectrophotometer. Then, the reliability of the full-wavelength microplate reader for detecting polysaccharide content was investigated and the measurement conditions were optimized. Finally, using this method to detect the polysaccharide content of natural plant poly-saccharide compound. The results showed that the full-wavelength microplate reader could replace the ultravio-let spectrophotometer to determine the polysaccharide content. For full-wavelength microplate reader method, the relative standard deviation ( RSD) value of precision was 0.41%, the RSD value of sample repeatability was 1.06%, the RSD value of standard repeatability was 0.84%, the recovery rate was 100.02%, the RSD value of sample stability was 1.25%, and the RSD value of standard stability was 1.34%. The main influencing factors including dilution multiple, detection wavelength, sulfuric acid addition and water bath temperature were screened. The determination conditions of polysaccharide content in compound polysaccharides of natural plant polysaccharide compound by full-wavelength microplate reader-phenol-sulfuric acid method were as fol-lows: sample was treated and diluted 200 times into a testing solution, determined as 5 mL of sulfuric acid, 30 ℃ water bath temperature, and 490 nm detection wavelength. Through measuring, the polysaccharide con-tent in the natural plant polysaccharide compound is 114. 70 mg/g. [ Chinese Journal of Animal Nutrition, 2019, 31(2) :922-929]关键词
全波长酶标仪/紫外分光光度计/苯酚-硫酸法/天然植物复方多糖Key words
full-wavelength microplate reader/ultraviolet spectrophotometer/phenol-sulfuric acid method/natural plant polysaccharide compound分类
农业科技引用本文复制引用
高倩,刘凤华,史雅然,李建东,邹闻书,金娜,陈长增..一种基于酶标仪检测天然植物复方多糖中多糖含量的方法[J].动物营养学报,2019,31(2):922-929,8.基金项目
北京市科技计划课题(D171100002117002) （D171100002117002）
公益性行业(农业)科研专项课题(201403051-07) （农业）
