医学分子生物学杂志2019，Vol.16Issue(1)：19-23,5.DOI:10. 3870/j. issn. 1672-8009. 2019. 01. 004
冬凌草甲素对人前列腺癌细胞系PC3增殖、凋亡和侵袭的调节作用
Regulatory Effect of Oridonin on the Proliferation, Apoptosis and In-vasion of Prostate Cancer Cell Line PC3
摘要
Abstract
Objective To investigate the effects of oridonin on the proliferation, apoptosis and invasion of prostate cancer cell line PC3. Methods Cells were divided into PC3, oridonin (5 m mol/L) , oridonin (10 mmol/L) and Oridonin (20 mmol/L) groups and treated with orido-nin at 0, 5, 10 and 20 mmol/L. Cell viability, apoptosis and invasion ability of PC3 cells was re-spectively detected by CCK8, flow cytometry and Transwell assy. The protein levels of Ki67, Caspase-3 and vascular endothelial growth factor ( VEGF ) were measured by Western blot-ting. Results Compared with PC3 group, the proliferation times of PC3 cells in Oridonin (5, 10, 20 mmol/L) group were decreased after treatment with oridonin for 4 days and the expression of pro-liferation-related protein Ki67 was inhibited significantly. Moreover, the cell apoptosis rate in Orido-nin (5, 10, 20 mmol/L) groups was increased as compared with PC3 group, and protein level of Caspase-3 was evidently increased. In addition, Oridonin (5, 10, 20 mmol/L) decreased inva-sion of PC3 cells and inhibited the expression of VEGF.关键词
前列腺癌/细胞增殖/细胞凋亡/侵袭Key words
prostate cancer/proliferation/apoptosis/invasion分类
医药卫生引用本文复制引用
LIU Shaoyong,YOU Lu,WANG Wei,ZHAI Changming..冬凌草甲素对人前列腺癌细胞系PC3增殖、凋亡和侵袭的调节作用[J].医学分子生物学杂志,2019,16(1):19-23,5.基金项目
重庆市自然科学基金(No.CSTC2006EB5030) （No.CSTC2006EB5030）
