医学分子生物学杂志2019，Vol.16Issue(1)：24-29,6.DOI:10. 3870/j. issn. 1672-8009. 2019. 01. 005
连翘苷对ACLT诱导的骨关节炎大鼠模型软骨组织损伤和TGF-β活性的调节作用
Regulatory Effect of Forsythin on Cartilage Tissue Damage and TGF-β Protein Activity in Rats with ACLT-induced Osteoarthritis
摘要
Abstract
Objective To study the effect of forsythin on the soft tissue damage and the activi-ty of transforming growth factor beta in rats with osteoarthritis induced by anterior cruciate ligament transaction ( ACLT) . Methods 20 male SD rats were randomly divided into 4 groups: healthy control group ( Ctrl) , forsythia solo alone group ( Forsythin) , model group ( OA model ) and model treatment group ( OA+Forsythin) , with 5 rats in each group. Osteoarthritis model was estab-lished by cutting off the anterior cruciate ligament. Intraperitoneal injection of 50 mg/kg BW forsyth-in was given once a day for 4 weeks. The cartilage damage was observed by using hematoxylin and e-osin staining, and the damage of soft tissue and fibrous hyperplasia by safranine O staining and MA-SON staining. The expression of matrix metalloproteinase 13, Ki67 and cysteine aspartic proteinase 3 was detected by Western blotting. Enzyme linked immunosorbent assay ( ELISA) was used to de-tect tumor necrosis factor alpha, interleukin 6, interleukin 10 and inducible nitric oxide synthase expression. Western blotting was used to detect the expression of TGF-beta 1, SMAD2 AND P-SMAD2. Results Compared with the control group, the cartilage tissue was obviously damaged and the fiber number increased in the OA group. The expression of MMP-13 and Caspase-3 was up signif-icantly; the expression of Ki67 was significantly down-regulated; the content of TNF-alpha, IL-6 and iNOS were increased significantly, the content of IL-10 was not significantly changed; the ex-pression of TGF-beta 1 was significantly up-regulated, and the p-Smad2/Smad2 ratio was signifi-cantly increased. Compared with the OA model group, in the OA+Forsythin group, the injury of cartilage tissue was obviously reduced and the fibrosis was obviously improved; the expression of MMP-13 and Caspase-3 was significantly down-regulated; the expression of Ki67 was significantly up-regulated; the content of TNF-alpha, IL-6 and iNOS decreased significantly; the content of IL-10 was not significantly changed; the expression of TGF-beta 1 was significantly down-regulated, and the p-Smad2/Smad2 ratio was decreased significantly. Conclusion Forsythin alleviates carti-lage damage and regulates TGF-beta activity in ACLT-induced osteoarthritis rats.关键词
连翘苷/骨关节炎/纤维增生/细胞凋亡Key words
forsythin/osteoarthritis/fibroplasia/apoptosis分类
医药卫生引用本文复制引用
ZHAO Zengtong,ZHU Xu,CHENG Wenhao,ZHANG Xinghuo,ZENG Jizhou..连翘苷对ACLT诱导的骨关节炎大鼠模型软骨组织损伤和TGF-β活性的调节作用[J].医学分子生物学杂志,2019,16(1):24-29,6.基金项目
北京市科技计划课题(No.z151100007879134) （No.z151100007879134）
