医学分子生物学杂志2019，Vol.16Issue(1)：57-61,5.DOI:10. 3870/j. issn. 1672-8009. 2019. 01. 011
miR-146a在乳腺癌组织中的表达及对乳腺癌细胞生物学特性的影响
Effect of miR-146a on Biological Behaviors of Breast Cancer Cells MDA-MB-231
摘要
Abstract
Objective To examine the expression and the pathological significance of miR-146 a in breast cancer and investigate the effects of its over-expression on the behavior of human breast cancer cell MDA-MB-231. Methods qRT-PCR was used to detect the expression of miR-146 a in 41 samples of human breast cancer tissues and 7 samples of normal tissues. We synthesized has-miR-146 a mimics, and then transfected them into MDA-MB-231 cells. The effect of miR-146 a was de-termined by real-time PCR. The cell growth and cell cycle of MDA-MB-231 cells were determined by flow cytometry. Results The expression of miR-146 a in breast cancer tissues was lower than in nor-mal tissues. The growth of MDA-MB-231 cells transfected with has-miR-146 a mimics was sup-pressed as compared with the control group. Conclusion miR-146 a is lowly expressed in breast cancer tissues and might be a useful biomarker for pathological identification of different types of breast cancer.关键词
乳腺癌/miR-146a/细胞增殖/细胞周期Key words
breast cancer/miR-146a/cell proliferation/cell cycle分类
医药卫生引用本文复制引用
ZHANG Yanping,LI Xuelan,DING Shi,CHEN Xingfeng..miR-146a在乳腺癌组织中的表达及对乳腺癌细胞生物学特性的影响[J].医学分子生物学杂志,2019,16(1):57-61,5.基金项目
湖南省教育厅科研项目(No.16C0205) （No.16C0205）
