山东农业大学学报（自然科学版）2019，Vol.50Issue(1)：19-24,6.DOI:10.3969/j.issn.1000-2324.2019.01.004
小麦和大麦GER4c基因启动子的分离及其锈菌诱导特性分析
The Wheat and Barley GER4c Promoter and Its Response to Rust Pathogen
摘要
Abstract
Pathogen-inducible promoter is a useful tool for studying plant-pathogen interaction and for molecular breeding, because such a promoter confers specific gene expression only when the plant pathogen presents. In order to identify rust pathogen inducible promoter in wheat and therefore provide basis for wheat rust-resistance research and breeding, we isolated two promoters of the Germin-like protein GER4c gene, one from barley and another from wheat. Expression vectors with the GFP fusion tag have been constructed and transformed into Brachypodium distachyon. Same expression pattern was found between the GER4c promoters of wheat and barley, of that the expression level of GFP was the highest in leaves and significantly lower in leaf sheaths, stems and spikelets. It was also found that both the wheat and barley GER4c promoters are rust pathogen inducible. When infected with Puccinia brachypodii isolate F-Fl, both the cDNA expression level and the fluorescent signal of the reporter gene GFP were significantly up-regulated in the leaves of transgenic Brachypodium. This study provides a rust pathogen inducible promoter to facilitate basic research and rust resistance breeding in wheat.关键词
诱导型启动子/锈菌/二穗短柄草/小麦/大麦/类萌发素蛋白Key words
Inducible promoter/ stripe rust/ Brachypodium distachyon/ wheat/ barley/ germin-like proteins分类
农业科技引用本文复制引用
黄德华1,张会飞1,刘强1,陈凤娟1,姜婷婷1,付道林2,吴佳洁1*..小麦和大麦GER4c基因启动子的分离及其锈菌诱导特性分析[J].山东农业大学学报（自然科学版）,2019,50(1):19-24,6.基金项目
国家重点研发计划子课题(2016YFD0101004) （2016YFD0101004）
国家转基因专项(2016ZX08009003-001-006) （2016ZX08009003-001-006）
