烟草科技2019，Vol.52Issue(1)：22-28,7.
洛阳烟区烟株根围抗烟草疫霉放线菌的筛选与鉴定
Screening and identification of antagonistic actinomycetes against Phytophthora parasitica from rhizospheric soils in Luoyang tobacco-growing areas
摘要
Abstract
To screen the antagonistic actinomycetes against Phytophthora parasitica from tobacco rhizospheric soils in order to control tobacco black shank,the antagonistic effects of actinomycetes from rhizospheric soils in Luoyang tobacco-growing areas and their fermentation broths against P. parasitica were determined by improved plate confrontation test and mycelial growth rate method. Dominant antagonistic strains were identified by morphological and biochemical features and 16S rDNA analysis. The results showed that 227 strains of actinomycetes were isolated,in which the inhibition rates of the fermentation broths of 8 strains against P. parasitica reached 100%,therefore the 8 strains were determined as the dominant antagonistic strains. The results of morphological and biochemical features and 16S rDNA analysis indicated that YY75,SX92 and SX59 were identified as Streptomyces lucensis,S. coralus and S. aureoverticillatus,respectively. YY124 and YY135 were identified as S. nobilis. LN204,LN221 and LN247 were identified as S. rochei. The 8 dominant strains all belonged to Actinobacteria,Actinobacteria,Stretomytales,Streptomycetaceae,Streptomyces.关键词
洛阳烟区/烟草/烟草疫霉/拮抗放线菌/形态鉴定/分子鉴定Key words
Luoyang tobacco-growing area/Tobacco/Phytophthora parasitica/Antagonistic actinomycete/Morphological identification/Molecular identification分类
农业科技引用本文复制引用
..洛阳烟区烟株根围抗烟草疫霉放线菌的筛选与鉴定[J].烟草科技,2019,52(1):22-28,7.基金项目
广西壮族自治区技术创新项目"植物颗粒滤棒减害增香技术彰显卷烟风格特征的研究及应用"(桂工信科技[2017]271号). （桂工信科技[2017]271号）
