烟草科技2019，Vol.52Issue(1)：60-66,7.
UPLC-MS/MS快速测定烟草中2,4-滴和2,4,5-涕
Rapid determination of 2,4?D and 2,4,5?T in tobacco by UPLC?MS/MS
摘要
Abstract
In order to analyze two common chlorinated phenoxy acid residues in tobacco, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for quantifying 2,4-D and 2,4,5-T was proposed. The analytes were extracted from tobacco samples by a hydrochloric acid solution and CH2Cl2. The extract was analyzed qualitatively and quantitatively by a tandem mass spectrometer in multiple reaction monitoring (MRM) mode after being separated on C18 column. The results showed that: 1) A good linear relation was obtained in the range of standard working solution with r>0.999. The limits of detection (LODs) and quantification (LOQs) were 0.004 5-0.007 6 and 0.013 8-0.023 6 mg/kg, respectively. The added standard recoveries of blank samples ranged from 79.5% to 94.6% with the relative standard deviations less than 6.0% . 2) The method was applied to 30 different types of tobacco samples, the results agreed well with those obtained by the tobacco industry standard method based on GC-MS. The proposed method is rapid and sensitive, no derivatization required, and consumes less organic reagents. It is suitable for the analysis of 2,4-D and 2,4,5-T in flue-cured tobacco, burley tobacco and oriental tobacco.关键词
烟草/2,4-滴/2,4,5-涕/超高效液相色谱-串联质谱/氯代苯氧羧酸类农药残留Key words
Tobacco/ 2,4-D/ 2,4,5-T/ Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/ Chlorinated phenoxy acid residue分类
农业科技引用本文复制引用
..UPLC-MS/MS快速测定烟草中2,4-滴和2,4,5-涕[J].烟草科技,2019,52(1):60-66,7.基金项目
湖南中烟工业有限责任公司细支烟研究重大专项项目"细支烟抽吸特性与调控方法研究"(KY2016JC0005). （KY2016JC0005）
