动物营养学报2019，Vol.31Issue(3)：1127-1137,11.DOI:10.3969/j.issn.1006-267x.2019.03.018
不同营养水平饲粮添加大豆酶解蛋白对蛋鸡生产性能、蛋品质、养分表观利用率及肠道形态的影响
Effects of Different Nutrient Level Diets Supplemented with Enzymolytic Soybean Meal on Performance, Egg Quality, Nutrient Apparent Availability and Intestinal Morphology of Laying Hens
摘要
Abstract
This experiment was conducted to investigate the effects of different nutrient level diets supplemen-ted with enzymolytic soybean meal ( ESM) on performance, egg quality, nutrient apparent availability and in-testinal morphology of laying hens. A 3×2 two factorial design with 3 nutrient levels in diet, positive control ( PC) with 11.22 MJ/kg metabolic energy ( ME) and 15.5% crude protein ( CP) , negative control 1 ( NC1) with 11.01 MJ/kg ME and 15.0% CP, negative control 2 ( NC2) with 10.80 MJ/kg ME and 14.5% CP, and two ESM supplemental levels ( 0 and 5 g/kg) was used. A total of 1 200 Roman pink laying hens with 52 weeks old were randomly divided into 6 groups with 10 replicates per group and 20 laying hens per replicate. The experiment lasted for 12 weeks. The results showed as follows: 1) with the decrease of nutrient levels in diet, average laying rate of laying hens had a decreasing tendency ( P=0.07) , and the ratio of feed to egg had an increasing tendency ( P=0.06) . With the increase of ESM supplemental levels, the ratio of feed to egg had a decreasing tendency ( P=0.05), and qualified egg rate was significantly increased ( P<0.05) . 2) With the decrease of nutrient levels in diet, yolk color had a decreasing tendency ( P=0.09) . With the increase of ESM supplemental levels, yolk color was significantly increased ( P<0.01) . The interaction of nutrient levels in diet and ESM supplemental levels had significant effects on albumen height and Haugh unit ( P<0.05) , NC1 nutri-ent level diets supplemented with ESM significantly increased albumen height and Haugh unit ( P<0.05) . 3) Apparent availability of ether extract ( EE) of laying hens in group PC was significantly higher than that of in groups NC1 and NC2 ( P<0.05) . With the increase of ESM supplemental levels, apparent availabilities of CP and EE were significantly increased ( P<0.05) . The interaction of nutrient levels in diet and ESM supplemental levels had significant effects on apparent availability of general energy of laying hens ( P<0.05), PC nutrient level diets supplemented with ESM significantly increased apparent availability of total energy ( P<0.05) . 4) With the decrease of nutrient levels in diet, crypt depth of jejunum had an increasing tendency ( P=0.06) , the ratio of villous height to crypt depth ( V/C) of jejunum was significantly decreased ( P<0.05) . With the in-crease of ESM supplemental levels, villous height of duodenum had an increasing tendency ( P=0.09) , V/C of duodenum was significantly increased ( P<0.05) . The interaction of nutrient levels in diet and ESM supple-mental levels had significant effects on villous height and V/C of jejunum of laying hens ( P<0.01) , NC2 nu-trient level diets supplemented with ESM significantly increased villous height and V/C of jejunum ( P<0.05) . In conclusion, diet supplemented with ESM can improve performance and egg quality of laying hens by impro-ving intestinal morphology and apparent availabilities of CP and EE in diet, and can partial alleviate the nega-tive influences by lower nutrient levels in diet. [ Chinese Journal of Animal Nutrition, 2019, 31 ( 3) :1127-1137]关键词
营养水平/大豆酶解蛋白/蛋鸡/蛋品质/生产性能/肠道形态Key words
nutrient levels/enzymolytic soybean meal/laying hens/egg quality/performance/intestinal morphology分类
农业科技引用本文复制引用
刘攀,王建萍,白世平,曾秋凤,丁雪梅,张克英..不同营养水平饲粮添加大豆酶解蛋白对蛋鸡生产性能、蛋品质、养分表观利用率及肠道形态的影响[J].动物营养学报,2019,31(3):1127-1137,11.基金项目
四川省重点研发计划(2018NZ0009) （2018NZ0009）
"十三五"育种攻关计划项目(2016NYZ0052) （2016NYZ0052）
