动物营养学报2019，Vol.31Issue(3)：1198-1209,12.DOI:10.3969/j.issn.1006-267x.2019.03.025
体外法研究延胡索酸对瘤胃甲烷、氢气产量和挥发性脂肪酸组成的影响
Effects of Fumarate on Ruminal Methane, Hydrogen Gas Production and Volatile Fatty Acid Composition Using an in Vitro Method
摘要
Abstract
This study was conducted to investigate the effects of different supplemental levels of fumarate on methane and hydrogen gas production, volatile fatty acids composition and microbial population through in vitro fermentation. Rumen fluid was collected from three adult Xiangdong black goats fitted with permanent ru-men cannulation. Using a 2×4 two-factor experimental design, corn flour and chicory were selected as sub-strates, fumarate had four (0, 3, 6 and 12 mmol/L) final concentrations, and blank groups were set for cor-recting the results, which were contained no substrate, just contained fumarate at concentrations of 0, 3, 6 or 12 mmol/L, respectively. Each group had 2 replicates, and were replicated 3 times. The 72 h in vitro ruminal incubation was performed using automatic fermentation incubation system. The results showed as follows: 1) the 72 h gas production and dry matter degradation of corn flour were significantly higher than those of chicory ( P<0.05) . Fumarate supplementation had greater 72 h gas production ( P<0.05) ; however, fumarate supple-mentation did not alter corrected 72 h gas production and dry matter degradation ( P>0.05) . There is no inter-action between substrates and fumarate on 72 h gas production and dry matter degradation ( P>0.05) . 2) Hy-drogen production and corrected hydrogen production of corn flour at 12 h were significantly higher than those of chicory ( P<0.05) , those at other time points did not have significant differences ( P>0.05) ; methane pro-duction and corrected methane production of corn flour at each time points were significantly higher than those of chicory ( P<0.05) . Fumarate had no significant effect on methane production, corrected methane produc-tion, hydrogen production and corrected hydrogen production at each time points ( P>0.05) . There is no inter-action between substrates and fumarate on hydrogen production and methane production ( P>0.05) . 3) Fumar-ate supplementation significantly increaed acetate, propionate and total volatile fatty acids concentrations ( P<0.05) , and significantly decreased pH and acetate to propionate ratio ( P<0.05) . But fumarate supplementation had no significant effect on corrected total volatile fatty acids concentrations( P>0.05) . 4) Fumarate supple-mentation did not significantly affect microbial population ( P>0.05) . This study indicates that corn flour con-tains more fermentable carbohydrates than chicory, which help produce more gas and volatile fatty acids. Fu-marate supplementation causes an increase in gas production and total volatile fatty acids concentration, which is mainly due to produce more gas and total volatile fatty acids during fumarate fermentation process, but has no significant effect on substrate self-degradation and microbial population.[ Chinese Journal of Animal Nutri-tion, 2019, 31(3) :1198-1209]关键词
延胡索酸/体外模拟瘤胃发酵/发酵类型/氢气产量/挥发性脂肪酸Key words
fumarate/in vitro simulated rumen fermentation/fermentation pattern/hydrogen production/vol-atile fatty acids分类
农业科技引用本文复制引用
王荣,文江南,王敏,邓近平,谭支良..体外法研究延胡索酸对瘤胃甲烷、氢气产量和挥发性脂肪酸组成的影响[J].动物营养学报,2019,31(3):1198-1209,12.基金项目
国家科技计划项目(2018YFD0501800,2016YFD050054) （2018YFD0501800,2016YFD050054）
国家自然科学基金项目(31561143009,31472133) （31561143009,31472133）
湖南省重大专项(2017NK1020) （2017NK1020）
中国科学院青年促进会项目(2016327) （2016327）
中国科学院特聘研究员项目(2018VBA0031) （2018VBA0031）
