动物营养学报2019，Vol.31Issue(3)：1278-1287,10.DOI:10.3969/j.issn.1006-267x.2019.03.033
抗生素诱导肠道菌群失调对肠黏膜屏障和肝脏功能的动态影响
Dynamic Effects of Antibiotic-Induced Intestinal Dysbacteriosis on Intestinal Mucosa Barrier and Liver Function
摘要
Abstract
In order to observe the dynamic changes of intestinal mucosa barrier and liver function for different degrees of intestinal dysbacteriosis models, mouse models were made which had intestinal dysbacteriosis caused by ceftriaxone sodium. After administration of ceftriaxone sodium for 3, 6 and 10 days, the intestinal contents were sampled to measure 16S rDNA gene, the histopathological changes of the ileum tissues were observed by hematoxylin-eosin ( HE) staining, the tight junction conditions of ileum tissues were observed by transmission electron microscope ( TEM) , the positive expression condition of zonula occluden-1 ( ZO-1) of ileum tissues was observed by immunohistochemical ( IHC) method, the contents of interleukin-1β ( IL-1β) and tumor nec-rosis factor-α ( TNF-α ) in in intestinal homogenate were assessed by enzyme-linked immunosorbent assay ( ELISA) method, the liver function indexes such as alanine aminotransferase ( ALT ) and aspartate amin-otransferase ( AST) activities in liver homogenate and histopathological changes of liver tissue were also evalu-ated. The results showed as follows: compared with the normal group, the relative abundances of Chryseobac-terium, Prevotella and Pseudomonas increased gradually, and the relative abundances of Bacillus, Lotus and Staphylococcus gradually decreased in model group after administration of ceftriaxone sodium for 3, 6 or 10 days. The positive expression of ZO-1 in ileum tissues was gradually decreased and the tight junction among the intestinal villus epithelial cells was gradually disappeared with the extension of ceftriaxone sodium treatment. Compared with the normal group, the contents of TNF-α and IL-1β in intestinal homogenate of model group had no changes at pre-dysbacteriosis ( after administration of ceftriaxone sodium for 3 and 6 days) , but in-creased significantly after the continued dysbacteriosis ( after administration of ceftriaxone sodium for 10 days) ( P<0.01) . Meanwhile, with the ongoing imbalance of intestinal flora, it could affect the liver, which showed the activities of ALT and AST in liver homogenate in model group were significantly higher than those in nor-mal group ( P<0. 01 ) , and hepatocyte swelling and disordered structure of hepatic lobule were obviously found. It is concluded that long-term intestinal dysbacteriosis can destroy the intestinal microecological balance, which is reflected in the contents of intestinal proinflammatory cytokines are gradually increased, and the liver function is also affected.[ Chinese Journal of Animal Nutrition, 2019, 31(3) :1278-1287]关键词
头孢曲松钠/肠道菌群失调/肠黏膜屏障/紧密连接/肝脏损伤Key words
ceftriaxone sodium/intestinal dysbacteriosis/intestinal mucosa barrier/tight junction/liver dam-age分类
医药卫生引用本文复制引用
李娜,赵玉民,陈正元,王碧君,罗霞,王青,何国林,熊天琴,桑传兰..抗生素诱导肠道菌群失调对肠黏膜屏障和肝脏功能的动态影响[J].动物营养学报,2019,31(3):1278-1287,10.基金项目
国家自然科学基金项目(81202997) （81202997）
广东省科技计划项目(2015A030302071) （2015A030302071）
广州市科技计划项目(201607010333) （201607010333）
