动物营养学报2019，Vol.31Issue(3)：1288-1294,7.DOI:10.3969/j.issn.1006-267x.2019.03.034
饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响
Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus
摘要
Abstract
This experiment was conducted to determine the effects of dietary pectic oligosaccharide ( POS) on the ileal barrier function of weaned pigs challenged by rotavirus ( RV ) . Twenty-eight healthy crossbred ( Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included POS treatment ( control diet or supplementing 200 mg/kg POS diet) and RV challenge ( oral gavage with RV or sterilized medium) . On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that RV infection increased the diarrhea rate and positive rate of rotavirus non-struc-tural protein 4 ( NSP4) in ileal mucosa, significantly decreased the villus height and villus height to crypt depth ratio of ileal mucosa ( P<0.05) , significantly reduced the expressions of zonula occludens-1 ( ZO-1) , occlu-din, mucin 1 and mucin 2 in ileal mucosa ( P<0.05) , significantly enhanced the malondialdehyde content in il-eal mucosa and E. coli population in ileal digesta ( P<0.05) , and significantly decreased total antioxidant ca-pacity in ileal mucosa and the population of Lactobacillus and total bacteria in ileal digesta of weaned pigs ( P<0.05) . Dietary POS reduced the diarrhea rate and positive rate of rotavirus NSP4 in ileal mucosa of the RV-in-fected weaned piglets, and significantly alleviated the effects of RV challenge on ileal mucosa morphology, mi-crobial population in ileal digesta, antioxidant capacity and expressions of occludin, mucin 1 and mucin 2 in il-eal mucosa of weaned piglets ( P<0.05) . These results suggest that dietary POS can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs. [ Chinese Journal of Animal Nutrition, 2019, 31(3) :1288-1294]关键词
果胶寡糖/断奶仔猪/轮状病毒/回肠屏障功能Key words
pectic oligosaccharide/weaned piglets/rotavirus/ileal barrier function分类
农业科技引用本文复制引用
毛湘冰,陈浩,陈代文,余冰,何军,郑萍,虞洁,罗钧秋,罗玉衡..饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J].动物营养学报,2019,31(3):1288-1294,7.基金项目
现代农业产业技术体系(CARS-35) （CARS-35）
四川省科技支撑项目(2016NYZ0052,2016NZ0006) （2016NYZ0052,2016NZ0006）
