动物营养学报2019，Vol.31Issue(3)：1396-1404,9.DOI:10.3969/j.issn.1006-267x.2019.03.047
高产纤维分解酶黑曲霉诱变选育与发酵条件优化
Mutagenesis Breeding and Fermentation Condition Optimization of High Yield Fiber Decomposition Enzyme of Aspergillus niger
摘要
Abstract
This experiment was conducted to study the mutagenesis breeding and optimal fermentation condition optimization of high yield fiber decomposition enzyme of Aspergillus niger. High cellulose decomposition en-zyme of mutant strain was obtained through the mutagenesis breeding ( ultraviolet, diethyl sulfate and ultravio-let-diethyl sulfate compound mutagenesis) of Aspergillus niger X1, and the fermentation conditions of enzyme production and the hydrolysis ability of straw were studied. The results showed that a mutant strain of Aspergil-lus niger X1U4-1 was selected through ultraviolet, diethyl sulfate and ultraviolet-diethyl sulfate compound mu-tagenesis of the original strain of Aspergillus niger X1; compared with the original strain, the filter paper en-zyme activity increased by 66.7%, the β-glucosidase activity increased by 22. 3%, the xylanase activity in-creased by 3.8% and the reducing sugar content (12 h) increased by 6.8% in mutant strain. The optimization of fermentation condition of Aspergillus niger X1U4-1 was that fermentation time was 84 h, corn kernel∶bran=3∶7, inoculation quantity was 1.2 mL, ammonium sulphate concentration was 4%; in this condi-tion, the filter paper enzyme activity reached 0.77 U/g, cellulase activity reached 93.80 U/g, xylanase activity reached 9 383.18 U/g. This study shows that the mutagenesis of ultraviolet-diethyl sulfuric acid is effective in mutagenesis breeding of Aspergillus niger, thus to improve enzyme producing ability and straw hydrolysis ca-pacity.[ Chinese Journal of Animal Nutrition, 2019, 31(3) :1396-1404]关键词
黑曲霉/紫外诱变/化学诱变/筛选/固态发酵Key words
Aspergillus niger/ultraviolet mutagenesis/chemical mutagenesis/screening/solid fermentation分类
农业科技引用本文复制引用
朱玉霞,张铁鹰,高爱琴,刘俊丽,朱玉杰,张志鹏,廖朝勇..高产纤维分解酶黑曲霉诱变选育与发酵条件优化[J].动物营养学报,2019,31(3):1396-1404,9.基金项目
中国农业科学院科技创新工程专项经费(ASTIP-IAS0) （ASTIP-IAS0）
