医学分子生物学杂志2019，Vol.16Issue(2)：132-136,142,6.DOI:10.3870/j.issn.1672-8009.2019.02.006
淫羊藿苷对人骨肉瘤细胞U2OS增殖、凋亡和迁移的调节作用
Effects of Icariin on Cell Proliferation, Apoptosis and Migration of Human Osteosarcoma Cell Line U2OS
摘要
Abstract
Objective To inveastigate the effect ofIcariinon cell proliferation, apoptosis and migration of human osteosarcoma cell line U2OS.Methods Cells were divided into U2OS group, Icariin (5 μmol/L) group, Icariin (10 μmol/L) group and Icariin (20 μmol/L) group.Cells were treated with icariin (at 0, 5, 10, 20 μmol/L) for 0, 1, 2, 3 and 4 d respectively, and cell viability was measured by CCK8 assay.Cells were treated with different doses of Icariin for 24 h.Flow cytometry was performed to determine cell apoptosis; the protein levels of Ki67, cleaved caspase-3 and matrix metalloproteinase-9 ( MMP-9) were measured by Western blotting.Results On the basis of preliminary study, 5 μmol/L, 10 μmol/L and 20 μmol/L were selected for further study.Compared with U2OS group, the growth of U2OS cells in Icariin (5 μmol/L) group, Icari- in (10 μmol/L) group and Icariin (20 μmol/L) group were decreased significantly, and the ex-pression level of Ki67 was down-regulated as compared with U2OS group.Meanwhile, the cell apop-tosis rates of cells in Icariin (5 μmol/L) , Icariin (10 μmol/L) and Icariin (20 μmol/L) groups were increased when compared with U2OS group, and the protein level of apoptosis-rated protein, cleaved caspase-3 in Icariin ( 5 μmol/L) , Icariin ( 10 μmol/L) and Icariin ( 20 μmol/L) groups was increased remarkedly compared with U2OS group.In addition, compared with U2OS group, the wound closure rate of cells and the expressions of migration-related protein, MMP-9 in Icariin (5 μmol/L) , Icariin (10 μmol/L) and Icariin (20 μmol/L) groups were decreased sig-nificantly.Conclusion Icariin can inhibit the proliferation and migration, and induce cell apopto-sis of human osteosarcoma cell line U2OS.关键词
骨肉瘤/ 细胞增殖/ 细胞凋亡/ 迁移Key words
osteosarcoma/ proliferation/ apoptosis/ migration分类
医药卫生引用本文复制引用
谭丽清,麦文秀,何文文..淫羊藿苷对人骨肉瘤细胞U2OS增殖、凋亡和迁移的调节作用[J].医学分子生物学杂志,2019,16(2):132-136,142,6.基金项目
广东省自然科学基金 (No.2017A030313904) This work was supported by a grant from the Natural Science Foundation of Guangdong Province (No.2017A0313904) （No.2017A030313904）
